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company name :
Rockland Immunochemicals
product type :
secondary antibody
product name :
Mouse IgG (H&L) Secondary Antibody Peroxidase Conjugated
catalog :
610-4302
quantity :
2
price :
169.00 USD
more info or order :
Rockland Immunochemicals product webpage
citations: 18
Reference
Amanat F, Clark J, Carre xf1 o J, Strohmeier S, Yellin T, Meade P, et al. Immunity to Seasonal Coronavirus Spike Proteins Does Not Protect from SARS-CoV-2 Challenge in a Mouse Model but Has No Detrimental Effect on Protection Mediated by COVID-19 mRNA Vaccination. J Virol. 2023;97:e0166422 pubmed publisher
Lim J, Bae J, Lee S, Lee D. Palmitoyl‑RGD promotes the expression of dermal‑epidermal junction components in HaCaT cells. Mol Med Rep. 2022;26: pubmed publisher
Amanat F, Strohmeier S, Meade P, Dambrauskas N, Muhlemann B, Smith D, et al. Vaccination with SARS-CoV-2 variants of concern protects mice from challenge with wild-type virus. PLoS Biol. 2021;19:e3001384 pubmed publisher
Amanat F, Thapa M, Lei T, Ahmed S, Adelsberg D, Carreño J, et al. SARS-CoV-2 mRNA vaccination induces functionally diverse antibodies to NTD, RBD, and S2. Cell. 2021;184:3936-3948.e10 pubmed publisher
Amanat F, Thapa M, Lei T, Sayed Ahmed S, Adelsberg D, Carreño J, et al. The plasmablast response to SARS-CoV-2 mRNA vaccination is dominated by non-neutralizing antibodies and targets both the NTD and the RBD. medRxiv. 2021;: pubmed publisher
Amanat F, Strohmeier S, Rathnasinghe R, Schotsaert M, Coughlan L, Garcia Sastre A, et al. Introduction of Two Prolines and Removal of the Polybasic Cleavage Site Lead to Higher Efficacy of a Recombinant Spike-Based SARS-CoV-2 Vaccine in the Mouse Model. MBio. 2021;12: pubmed publisher
Jiang C, Moorthy B, Patel D, Kumar A, Morgan W, Alfonso B, et al. Regulation of Mitochondrial Respiratory Chain Complex Levels, Organization, and Function by Arginyltransferase 1. Front Cell Dev Biol. 2020;8:603688 pubmed publisher
Wakabayashi R, Kono H, Kozaka S, Tahara Y, Kamiya N, Goto M. Transcutaneous Codelivery of Tumor Antigen and Resiquimod in Solid-in-Oil Nanodispersions Promotes Antitumor Immunity. ACS Biomater Sci Eng. 2019;5:2297-2306 pubmed publisher
Amanat F, Strohmeier S, Rathnasinghe R, Schotsaert M, Coughlan L, Garcia Sastre A, et al. Introduction of two prolines and removal of the polybasic cleavage site leads to optimal efficacy of a recombinant spike based SARS-CoV-2 vaccine in the mouse model. bioRxiv. 2020;: pubmed publisher
Amanat F, White K, Miorin L, Strohmeier S, McMahon M, Meade P, et al. An In Vitro Microneutralization Assay for SARS-CoV-2 Serology and Drug Screening. Curr Protoc Microbiol. 2020;58:e108 pubmed publisher
Wang T, Coppens I, Saorin A, Brady N, Hamacher Brady A. Endolysosomal Targeting of Mitochondria Is Integral to BAX-Mediated Mitochondrial Permeabilization during Apoptosis Signaling. Dev Cell. 2020;53:627-645.e7 pubmed publisher
Fegan J, Calmettes C, Islam E, Ahn S, Chaudhuri S, Yu R, et al. Utility of Hybrid Transferrin Binding Protein Antigens for Protection Against Pathogenic Neisseria Species. Front Immunol. 2019;10:247 pubmed publisher
Kirkpatrick E, Qiu X, Wilson P, Bahl J, Krammer F. The influenza virus hemagglutinin head evolves faster than the stalk domain. Sci Rep. 2018;8:10432 pubmed publisher
Platt T, Beckett T, Kohler K, Niedowicz D, Murphy M. Obesity, diabetes, and leptin resistance promote tau pathology in a mouse model of disease. Neuroscience. 2016;315:162-74 pubmed publisher
Beckett T, Webb R, Niedowicz D, Holler C, Matveev S, Baig I, et al. Postmortem Pittsburgh Compound B (PiB) binding increases with Alzheimer's disease progression. J Alzheimers Dis. 2012;32:127-38 pubmed
O Shannessy D, Somers E, Albone E, Cheng X, Park Y, Tomkowicz B, et al. Characterization of the human folate receptor alpha via novel antibody-based probes. Oncotarget. 2011;2:1227-43 pubmed
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product information
Catalog Number :
610-4302
Name :
Rabbit Anti-Mouse IgG (H&L) Antibody Peroxidase Conjugated - 610-4302
Display Name :
Mouse IgG (H&L) Secondary Antibody Peroxidase Conjugated
Application Note :
Anti-Mouse secondary antibody conjugated to horseradish peroxidase (HRP) generated in rabbit detects specifically Mouse IgG whole molecule. Anti-Mouse IgG peroxidase antibody has been tested by ELISA and western blot and is suitable for ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation, Immunohistochemistry as well as other HRP antibody based assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
2.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
HRP
Size :
2
Default Unit :
mg
ELISA Dilution :
1:50,000 - 1:300,000
Immunohistochemistry Dilution :
1:500 - 1:2,500
Western Blot Dilution :
1:10,000 - 1:30,000
Expiration :
Expiration date is one (1) year from date of receipt.
Format :
IgG
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Mouse IgG whole molecule
Packing Type :
Ambient
Physical State :
Lyophilized
Preservative :
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Purity and Specificity :
HRP Secondary Antibody Conjugate was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
Reconstitution Buffer :
Restore with deionized water (or equivalent)
Reconstitution Volume :
1.0 mL
Species Reactivity :
Mouse
Tested Applications :
ELISA; WB
Suggested Applications :
IF; Other
Stabilizer :
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Storage :
Store mouse secondary antibody conjugate at 4° C prior to restoration.   For extended storage aliquot antibody and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Synonyms :
Rabbit Anti-Mouse IgG Secondary Antibody Peroxidase Conjugated, Rabbit Anti-Mouse IgG Secondary Antibody HRP Conjugated, RAM-HRP, Anti-mouse secondary antibody, anti-mouse HRP antibody, horseradish peroxidase conjugated secondary antibody, anti-mouse horseradish peroxidase conjugated secondary antibody
Target Species :
Mouse
Background :
Secondary antibodies bind to the primary antibody to assist in detection, sorting and purification of target antigens. To enable detection, the secondary antibody must have specificity for the antibody species and isotype of the primary antibody being used and generally is conjugated. Rockland produces highly active antibodies and conjugates against mouse immunoglobulins. Anti-Mouse Secondary Antibodies are affinity-purified polyclonal antibodies with well-characterized specificity for mouse immunoglobulin classes, subclasses, and fragments. They are available as unlabeled antibodies as well as conjugates of alkaline phosphatase (AP), horseradish peroxidase (HRP), fluorescent conjugates, and biotin. This Anti-MOUSE IgG generated in rabbit is designed to detect heavy and light chains (H&L) and has been Peroxidase (HRP) conjugated. Rigorous quality control testing ensures that the finished product meets or exceeds out high standards for optimum performance in your assays. These secondary antibodies are used throughout various types of assays, including ELISA or Western Blot, Immunohistochemistry, Flow Cytometry. Optimal secondary antibody requires knowledge of the detection assay.
Low Endotoxin :
No
Sample Size :
No
Secondary Antibody Specificity :
IgG (H&L)
Application Text :
ELISA,Immunohistochemistry,Western Blot,
Other :
User Optimized
Category :
Secondary Antibodies
Conjugation Name :
Peroxidase (HRP)
2025 Price :
169.00 USD
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.