Mouse IgG (H&L) Antibody Peroxidase Conjugated Pre-adsorbed | Rockland Immunochemicals 610-103-121 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Mouse IgG (H&L) Antibody Peroxidase Conjugated Pre-adsorbed

catalog :

610-103-121

quantity :

price :

284.00 USD

clonality :

polyclonal

host :

domestic goat

conjugate :

HRP

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

citations: 19

Reference
Wang W, Taufalele P, Millet M, Homsy K, Smart K, Berestesky E, et al. Matrix stiffness regulates tumor cell intravasation through expression and ESRP1-mediated alternative splicing of MENA. Cell Rep. 2023;42:112338 pubmed publisher
Schenke M, Prause H, Bergforth W, Przykopanski A, Rummel A, Klawonn F, et al. Human-Relevant Sensitivity of iPSC-Derived Human Motor Neurons to BoNT/A1 and B1. Toxins (Basel). 2021;13: pubmed publisher
Yingling C, Pruyne D. FHOD formin and SRF promote post-embryonic striated muscle growth through separate pathways in C. elegans. Exp Cell Res. 2021;398:112388 pubmed publisher
Schöttelndreier D, Langejürgen A, Lindner R, Genth H. Low Density Lipoprotein Receptor-Related Protein-1 (LRP1) Is Involved in the Uptake of Clostridioides difficile Toxin A and Serves as an Internalizing Receptor. Front Cell Infect Microbiol. 2020;10:565465 pubmed publisher
Timón Gómez A, Pérez Pérez R, Nyvltová E, Ugalde C, Fontanesi F, Barrientos A. Protocol for the Analysis of Yeast and Human Mitochondrial Respiratory Chain Complexes and Supercomplexes by Blue Native Electrophoresis. STAR Protoc. 2020;1: pubmed publisher
Kim M, Wu M, Zhang Y, Yang J, Chang C. TET2 directs mammary luminal cell differentiation and endocrine response. Nat Commun. 2020;11:4642 pubmed publisher
Amanat F, Stadlbauer D, Strohmeier S, Nguyen T, Chromikova V, McMahon M, et al. A serological assay to detect SARS-CoV-2 seroconversion in humans. Nat Med. 2020;: pubmed publisher
Timón Gómez A, Garlich J, Stuart R, Ugalde C, Barrientos A. Distinct Roles of Mitochondrial HIGD1A and HIGD2A in Respiratory Complex and Supercomplex Biogenesis. Cell Rep. 2020;31:107607 pubmed publisher
Yang X, He Z, Zhang G, Lü J, Zhang H, Ren H, et al. Evaluation of Reactivity of Monoclonal Antibodies Against Omp25 of Brucella spp. Front Cell Infect Microbiol. 2020;10:145 pubmed publisher
Nishida T, Nagao Y, Hashitani S, Yamanaka N, Takigawa M, Kubota S. Suppression of adipocyte differentiation by low-intensity pulsed ultrasound via inhibition of insulin signaling and promotion of CCN family protein 2. J Cell Biochem. 2020;: pubmed publisher
Genth H, Junemann J, Lämmerhirt C, Lücke A, Schelle I, Just I, et al. Difference in Mono-O-Glucosylation of Ras Subtype GTPases Between Toxin A and Toxin B From Clostridioides difficile Strain 10463 and Lethal Toxin From Clostridium sordellii Strain 6018. Front Microbiol. 2018;9:3078 pubmed publisher
Lobo Jarne T, Nyvltová E, Pérez Pérez R, Timón Gómez A, Molinié T, Choi A, et al. Human COX7A2L Regulates Complex III Biogenesis and Promotes Supercomplex Organization Remodeling without Affecting Mitochondrial Bioenergetics. Cell Rep. 2018;25:1786-1799.e4 pubmed publisher
Schöttelndreier D, Seeger K, Grassl G, Winny M, Lindner R, Genth H. Expression and (Lacking) Internalization of the Cell Surface Receptors of Clostridioides difficile Toxin B. Front Microbiol. 2018;9:1483 pubmed publisher
Brown C, Dayan S, Wong S, Kaczmarek A, Hope C, Pederson S, et al. FOXP3 and miR-155 cooperate to control the invasive potential of human breast cancer cells by down regulating ZEB2 independently of ZEB1. Oncotarget. 2018;9:27708-27727 pubmed publisher
Fan L, Tan B, Li Y, Zhao Q, Yuan H, Liu Y, et al. Upregulation of miR‑185 promotes apoptosis of the human gastric cancer cell line MGC803. Mol Med Rep. 2018;17:3115-3122 pubmed publisher
Langer N, Steinicke F, Lindigkeit R, Ernst L, Beuerle T. Determination of cross-reactivity of poly- and monoclonal antibodies for synthetic cannabinoids by direct SPR and ELISA. Forensic Sci Int. 2017;280:25-34 pubmed publisher
Shen H, Li L, Xue K, Hu D, Gao Y. Antitumor activity of fucoidan in anaplastic thyroid cancer via apoptosis and anti-angiogenesis. Mol Med Rep. 2017;15:2620-2624 pubmed publisher
Ercan E, Han J, Di Nardo A, Winden K, Han M, Hoyo L, et al. Neuronal CTGF/CCN2 negatively regulates myelination in a mouse model of tuberous sclerosis complex. J Exp Med. 2017;214:681-697 pubmed publisher
Ciesielska A, Sas Nowosielska H, Kwiatkowska K. Bis(monoacylglycero)phosphate inhibits TLR4-dependent RANTES production in macrophages. Int J Biochem Cell Biol. 2017;83:15-26 pubmed publisher

image

image 1 :

Western Blot of Peroxidase conjugated Goat anti-Mouse IgG antibody. Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase mouse secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 28 kDa for Mouse IgG. Other band(s): none.

product information

Catalog Number :

610-103-121

Name :

Goat Anti-Mouse IgG (H&L) Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins) - 610-103-121

Display Name :

Mouse IgG (H&L) Antibody Peroxidase Conjugated Pre-adsorbed

Application Note :

Anti-Mouse IgG Peroxidase Antibody has been tested by ELISA and western blot and is ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

HRP

Size :

Default Unit :

ELISA Dilution :

1:180,000

Immunohistochemistry Dilution :

1:1,000 - 1:5,000

Western Blot Dilution :

1:2,000 - 1:20,000

Expiration :

Expiration date is one (1) year from date of receipt.

Format :

IgG

Host Animal :

Goat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Mouse IgG whole molecule

Packing Type :

Ambient

Physical State :

Lyophilized

Preservative :

0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Purity and Specificity :

HRP secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

Reconstitution Buffer :

Restore with deionized water (or equivalent)

Reconstitution Volume :

1.0 mL

Species Reactivity :

Mouse

Tested Applications :

ELISA; WB

Suggested Applications :

IHC; Other

Stabilizer :

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Storage :

Store secondary antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Anti-Mouse IgG HRP secondary antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

Goat Anti-Mouse IgG Secondary Antibody Peroxidase Conjugated, Goat Anti-Mouse IgG Secondary Antibody HRP Conjugated, GAM-HRP, Anti-mouse secondary antibody, anti-mouse HRP antibody, horseradish peroxidase conjugated secondary antibody, anti-mouse horseradish peroxidase conjugated secondary antibody

Target Species :

Mouse

Background :

Anti-Mouse IgG Peroxidase Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Low Endotoxin :

Sample Size :

Secondary Antibody Specificity :

IgG (H&L)

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Category :

Secondary Antibodies

Conjugation Name :

Peroxidase (HRP)

2025 Price :

284.00 USD

more info or order :

Rockland Immunochemicals product webpage