Mouse IgG (H&L) Secondary Antibody Fluorescein Conjugated Pre-Adsorbed | Rockland Immunochemicals 610-102-121 product information

product summary

company name :

Rockland Immunochemicals

product type :

secondary antibody

product name :

Mouse IgG (H&L) Secondary Antibody Fluorescein Conjugated Pre-Adsorbed

catalog :

610-102-121

quantity :

1 mg

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

610-102-121

Name :

Anti-MOUSE IgG (H&L) (GOAT) Antibody Fluorescein Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins) - 610-102-121

Display Name :

Mouse IgG (H&L) Secondary Antibody Fluorescein Conjugated Pre-Adsorbed

Application Note :

Secondary antibody reagents are ideal for Fluorescent Western Blot, FLISA, Flow Cytometry, Immunohistochemistry and Immunofluorescence Microscopy as well as other antibody detection methods.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

FITC

Size :

1 mg

Default Unit :

FLISA Dilution :

1:10,000 - 1:50,000

Flow Cytometry Dilution :

1:500 - 1:2,500

Fluorochrome Protein Value :

3.08

Immunohistochemistry Dilution :

User Optimized

IF Microscopy Dilution :

1:1,000 - 1:5,000

Western Blot Dilution :

User Optimized

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Host Animal :

Goat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Mouse IgG whole molecule

Packing Type :

Ambient

Physical State :

Lyophilized

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Goat Anti-Mouse IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

Reconstitution Buffer :

Restore with deionized water (or equivalent)

Reconstitution Volume :

1.0 mL

Species Reactivity :

Mouse

Stabilizer :

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Storage :

Store anti mouse secondary antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

Goat Anti-Mouse IgG Secondary Antibody fluorescein Conjugated, Goat Anti-Mouse IgG Antibody FITC Conjugated, GAM-FITC, Anti-mouse IgG secondary antibody, anti-mouse IgG Fluorescein conjugated secondary antibody

Target Species :

Mouse

Background :

Anti-Mouse IgG Fluorescein Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Low Endotoxin :

Sample Size :

Secondary Antibody Specificity :

IgG (H&L)

Application Text :

FLISA,Flow Cytometry,Immunohistochemistry,IF Microscopy,Western Blot,

Category :

Secondary Antibodies

Conjugation Name :

Fluorescein (FITC)

Primary Image Name :

Fluorescein Goat Anti Mouse IgG (H&L) Antibody – Dot Blot

more info or order :

Rockland Immunochemicals product webpage