Mouse IgG2a Antibody Fluorescein Conjugated Pre-adsorbed | Rockland Immunochemicals 610-102-041 product information

product summary

company name :

Rockland Immunochemicals

product type :

secondary antibody

product name :

Mouse IgG2a Antibody Fluorescein Conjugated Pre-adsorbed

catalog :

610-102-041

quantity :

1 mg

more info or order :

Rockland Immunochemicals product webpage

citations: 3

Reference
Carlyle B, Kitchen R, Kanyo J, Voss E, Pletikos M, Sousa A, et al. A multiregional proteomic survey of the postnatal human brain. Nat Neurosci. 2017;20:1787-1795 pubmed publisher
Musante V, Li L, Kanyo J, Lam T, Colangelo C, Cheng S, et al. Reciprocal regulation of ARPP-16 by PKA and MAST3 kinases provides a cAMP-regulated switch in protein phosphatase 2A inhibition. elife. 2017;6: pubmed publisher
Andrade E, Musante V, Horiuchi A, Matsuzaki H, Brody A, Wu T, et al. ARPP-16 Is a Striatal-Enriched Inhibitor of Protein Phosphatase 2A Regulated by Microtubule-Associated Serine/Threonine Kinase 3 (Mast 3 Kinase). J Neurosci. 2017;37:2709-2722 pubmed publisher

product information

Catalog Number :

610-102-041

Name :

Anti-MOUSE IgG2a (Gamma 2a chain) (GOAT) Antibody Fluorescein Conjugated (Min Cross Bv, Hu, and Rb Serum Proteins) - 610-102-041

Display Name :

Mouse IgG2a Antibody Fluorescein Conjugated Pre-adsorbed

Application Note :

Mouse IgG2a secondary antibody is available in a variety of formats. FITC Conjugated Secondary Antibodies are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.7 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

FITC

Size :

1 mg

Default Unit :

FLISA Dilution :

1:10,000 - 1:50,000

Flow Cytometry Dilution :

1:200 - 1:500

Fluorochrome Protein Value :

5.2

Immunohistochemistry Dilution :

1:1,000 - 1:5,000

IF Microscopy Dilution :

1:1,000 - 1:5,000

Western Blot Dilution :

1:2,000 - 1:10,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Host Animal :

Goat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Mouse IgG2a heavy chain

Packing Type :

Ambient

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

MOUSE IgG2a Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Goat Serum, Mouse Serum and Mouse IgG2a. Specificity was confirmed by ELISA at less than 1% cross-reactivity against other mouse heavy or light chain isotypes. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.

Reconstitution Buffer :

Restore with deionized water (or equivalent)

Reconstitution Volume :

1.0 mL

Species Reactivity :

Mouse

Stabilizer :

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Storage :

Store anti mouse secondary antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

goat anti-MOUSE IgG2a Antibody fluorescein Conjugation, goat anti-Mouse IgG2a (gamma 2a) FITC Conjugated Antibody

Target Species :

Mouse

Background :

Anti-Mouse IgG2a Fluorescein Antibody generated in goat detects reactivity to Mouse IgG2a (Gamma 2a chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25% of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Immunogen Type :

Native Protein

Low Endotoxin :

Sample Size :

Secondary Antibody Specificity :

IgG2a

Application Text :

FLISA,Flow Cytometry,Immunohistochemistry,IF Microscopy,Western Blot,

Other :

User Optimized

Category :

Secondary Antibodies

Conjugation Name :

Fluorescein (FITC)

Primary Image Name :

Mouse IgG2a Antibody Fluorescein Conjugated Pre-absorbed - Dot Blot

more info or order :

Rockland Immunochemicals product webpage