Beta Catenin phospho S33/phospho S37 Antibody | Rockland Immunochemicals 609-401-D12 product information

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product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Beta Catenin phospho S33/phospho S37 Antibody

catalog :

609-401-D12

quantity :

100 µL

price :

366 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA

product information

Catalog Number :

609-401-D12

Name :

Anti-beta Catenin pS33/pS37 (Rabbit) Antibody - 609-401-D12

Display Name :

Beta Catenin phospho S33/phospho S37 Antibody

Application Note :

Anti-beta Catenin pS33/pS37 antibody is suitable for use in ELISA, Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 83 kDa in size corresponding to the beta catenin protein phosphorylated at Ser33 and 37 in the appropriate cell lysate or extract.

Buffer :

0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Clonality :

Polyclonal

Concentration Value :

Titrated value sufficient to run approximately 10 mini blots.

Conjugation :

(None)

Size :

100 µL

Default Unit :

ELISA Dilution :

1:10,000

Western Blot Dilution :

1:1,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

CTNNB1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-Beta Catenin pS33/pS37 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser33/37.

Packing Type :

Dry Ice

Physical State :

Liquid

Purity and Specificity :

Anti-Beta Catenin pS33/pS37 Antibody is directed against beta human Catenin protein phosphorylated ar S33/pS37. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated beta Catenin. Minimal reactivity occurs against non-phosphorylated beta Catenin. Reactivity against beta Catenin occurs from human sources. However, reactivity is also expected against mouse, rat and Xenopus based on 100% sequence homology. Cross reactivity from other sources has not been determined.

Species Reactivity :

human

Stabilizer :

0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol

Storage :

Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Synonyms :

rabbit anti-Beta Catenin pS33/pS37 Antibody, Catenin beta-1, beta catenin, ß-catenin, CTNNB1, CTNNB, beta catenin antibody, anti-beta catenin

Target Species :

Human

Background :

Beta Catenin antibody recognizes β-catenin which is a central component of the cadherin cell adhesion complex and plays an essential role in neural development in the Wingless/Wnt signaling pathway. The role of β-catenin in these processes is thought regulated by the sequential phosphorylation of Ser29 Ser33, Ser37 and Thr41 by glycogen synthase kinase 3β (GSK3β). This hyperphosphorylation promotes the ubiquitylation and targeted destruction of β-catenin. Mutations in components of this phosphorylation regulated process that prevent β-catenin hyperphosphorylation by GSK3β are trongly associated with cancers. Anti-Beta Catenin antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

General Reference :

Ding Y and Dale, T (2002). Wnt signal transduction: kinase cogs in a nano-machine? Trends Biochem Sci 27:327-329. Liu C, Li Y, Semenov M, Han C, Baeg GH, Tan Y, Zhang Z, Lin X and He X (2002). Control of β-catenin phosphorylation/degradation by a dual-kinase mechanism. Cell 108:837-847. Polakis P (2000). Wnt signaling and cancer. Genes Dev 14:1837-1851. Wang ZH, Vogelstein B and Kinzler KW (2003). Phosphorylation of β-catenin at S33, S37, or T41 can occur in the absence of phosphorylation at T45 in colon cancer cells. Cancer Res 63:5234-5235.

Immunogen Type :

Peptide

Low Endotoxin :

Post Translational Modification :

Phosphorylation

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2020 Price :

366 USD

UniProt :

P35222

Primary Image Name :

Western Blot of Anti-beta Catenin pS33/pS37 (Rabbit) Antibody - 609-401-D12