Human IgG (H&L) Antibody Peroxidase Conjugated | Rockland Immunochemicals 609-1302 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Human IgG (H&L) Antibody Peroxidase Conjugated

catalog :

609-1302

quantity :

2.0

price :

185.00 USD

clonality :

polyclonal

host :

domestic goat

conjugate :

HRP

application :

ELISA, dot blot

more info or order :

Rockland Immunochemicals product webpage

citations: 3

Reference
Phan I, Subramanian S, Kim D, Murphy M, Pettie D, Carter L, et al. In silico detection of SARS-CoV-2 specific B-cell epitopes and validation in ELISA for serological diagnosis of COVID-19. Sci Rep. 2021;11:4290 pubmed publisher
Jian L, Xiujian S, Yuangang Y, Yan X, Lianchao Y, Duthie M, et al. Evaluation of antibody detection against the NDO-BSA, LID-1 and NDO-LID antigens as confirmatory tests to support the diagnosis of leprosy in Yunnan province, southwest China. Trans R Soc Trop Med Hyg. 2020;114:193-199 pubmed publisher
Serrano Coll H, Muñoz M, Camilo Beltrán J, Duthie M, Cardona Castro N. Anti-natural octyl disaccharide-leprosy IDRI diagnostic (NDO-LID) antibodies as indicators of leprosy reactions and neuritis. Trans R Soc Trop Med Hyg. 2017;111:125-131 pubmed publisher

product information

Catalog Number :

609-1302

Name :

Goat Anti-Human IgG (H&L) Antibody Peroxidase Conjugated - 609-1302

Display Name :

Human IgG (H&L) Antibody Peroxidase Conjugated

Application Note :

Anti-Human IgG (H&L) peroxidase conjugated antibody has been tested by dot blot and ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

2.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

HRP

Size :

2.0

Default Unit :

ELISA Dilution :

1:20,000 - 1:80,000

Immunohistochemistry Dilution :

1:1,000 - 1:5,000

Western Blot Dilution :

1:5,000 - 1:25,000

Expiration :

Expiration date is one (1) year from date of receipt.

Format :

IgG

Host Animal :

Goat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-Human IgG (H&L) was produced by repeated immunization with human IgG whole molecule in goat.

Packing Type :

Ambient

Physical State :

Lyophilized

Preservative :

0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Purity and Specificity :

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG and Human Serum.

Reconstitution Buffer :

Restore with deionized water (or equivalent)

Species Reactivity :

Human

Tested Applications :

Dot Blot; ELISA

Stabilizer :

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Storage :

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

goat anti-Human IgG peroxidase conjugated Antibody, goat anti-Human IgG Antibody HRP conjugation

Target Species :

Human

Background :

Anti-Human IgG (H&L) peroxidase conjugated antibody generated in goat detects specifically human IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This anti-Human peroxidase conjugated secondary antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Immunogen Type :

Native Protein

Low Endotoxin :

Sample Size :

Secondary Antibody Specificity :

IgG (H&L)

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Other :

User Optimized

Category :

Secondary Antibodies

Conjugation Name :

Peroxidase (HRP)

2025 Price :

185.00 USD

more info or order :

Rockland Immunochemicals product webpage