product summary
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company name :
Rockland Immunochemicals
product type :
secondary antibody
product name :
Human IgG IgA IgM (H&L) Antibody Peroxidase Conjugated
catalog :
609-103-130
quantity :
2 mg
citations: 1
Reference
Crawford L, Tempel R, Streblow D, Kreklywich C, Smith P, Picker L, et al. Human Cytomegalovirus Induces Cellular and Humoral Virus-specific Immune Responses in Humanized BLT Mice. Sci Rep. 2017;7:937 pubmed publisher
product information
Catalog Number :
609-103-130
Name :
Anti-HUMAN IgG IgA IgM (H&L) (GOAT) Antibody Peroxidase Conjugated - 609-103-130
Display Name :
Human IgG IgA IgM (H&L) Antibody Peroxidase Conjugated
Application Note :
Anti-Human IgG IgA IgM (H&L) Peroxidase Antibody is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
2.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
HRP
Size :
2 mg
Default Unit :
mg
ELISA Dilution :
1:20,000 - 1:100,000
Immunohistochemistry Dilution :
1:1,000 - 1:5,000
Immunoprecipitation Dilution :
1:1,000 - 1:5,000
Western Blot Dilution :
1:2,000 - 1:10,000
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG
Host Animal :
Goat
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-Human IgG IgA IgM (H&L) was produced by repeated immunization with Human IgG, IgA and IgM whole molecules in goat.
Packing Type :
Ambient
Physical State :
Lyophilized
Preservative :
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Purity and Specificity :
This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG, Human IgA and Human IgM.  This reagent is suitable for the detection of all Human immunoglobulin subclasses and chain combinations.
Reconstitution Buffer :
Restore with deionized water (or equivalent)
Reconstitution Volume :
1.0 mL
Stabilizer :
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Storage :
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Synonyms :
Goat Anti-Human IgG IgA IgM Antibody peroxidase Conjugated, Goat Anti-Human IgG IgA IgM Antibody peroxidase Conjugated, Goat Anti-Human IgGAM Antibody HRP Conjugated
Target Species :
Human
Background :
Anti-Human IgG IgA IgM (H&L) Peroxidase Antibody generated in goat detects human (heavy and light chain) immunoglobulin G, A, and M. Immunoglobulin G binds to antigens and can neutralize or opsinize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Immunogen Type :
Native Protein
Low Endotoxin :
No
Sample Size :
No
Secondary Antibody Specificity :
IgG IgA IgM
Application Text :
ELISA,Immunohistochemistry,Immunoprecipitation,Western Blot,
Other :
User Optimized
Category :
Secondary Antibodies
Conjugation Name :
Peroxidase (Horseradish)
Primary Image Name :
Human IgG IgA IgM (H&L) Antibody Peroxidase Conjugated - Western Blot
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.