Bovine IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated | Rockland Immunochemicals 601-1504 product information

product summary

company name :

Rockland Immunochemicals

product type :

secondary antibody

product name :

Bovine IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated

catalog :

601-1504

quantity :

1 mg

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

601-1504

Name :

Anti-BOVINE IgG F(ab')2 (GOAT) Antibody Alkaline Phosphatase Conjugated - 601-1504

Display Name :

Bovine IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated

Application Note :

This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,500 is suggested for this product.

Buffer :

0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

ALP

Size :

1 mg

Default Unit :

ELISA Dilution :

1:2,000 - 1:10,000

Immunohistochemistry Dilution :

1:200 - 1:1,000

Western Blot Dilution :

1:500 - 1:2,500

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Host Animal :

Goat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Bovine IgG F(ab')2 fragment

Packing Type :

Wet Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.05% (w/v) Sodium Azide

Purity and Specificity :

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Bovine IgG, Bovine IgG F(ab’)2 and Bovine Serum. No reaction was observed against Bovine IgG F(c).

Stabilizer :

10 mg/ml Polyethylene Glycol (PEG-8000)

Storage :

Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Synonyms :

goat anti-Bovine IgG F(ab')2 fragment alkaline phosphatase Conjugated Antibody, goat anti-Bovine IgG Fab2 fragment Alk phos Conjugated Antibody

Target Species :

Bovine

Background :

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.

Low Endotoxin :

Sample Size :

Secondary Antibody Specificity :

IgG F(ab')2

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Other :

User Optimized

Category :

Secondary Antibodies

Conjugation Name :

Alkaline Phosphatase (Calf Intestine)

more info or order :

Rockland Immunochemicals product webpage