DYKDDDDK Antibody same epitope as Sigma's Anti-FLAG Dylight™ 800 Conjugated | Rockland Immunochemicals 600-445-383 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

DYKDDDDK Antibody same epitope as Sigma's Anti-FLAG Dylight™ 800 Conjugated

catalog :

600-445-383

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

DyLight800

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-445-383

Name :

Antibody for the detection of FLAG™ conjugated proteins (RABBIT) Antibody DyLight™ 800 Conjugated - 600-445-383

Display Name :

DYKDDDDK Antibody same epitope as Sigma's Anti-FLAG Dylight™ 800 Conjugated

Application Note :

The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

DL800

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000 - 1:25,000

FLISA Dilution :

>1:20,000

Fluorochrome Protein Value :

2.0

IF Microscopy Dilution :

>1:5,000

Western Blot Dilution :

1:10,000 - 1:25,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This affinity purified antibody was purified from whole rabbit serum prepared by repeated immunizations with the Enterokinase Cleavage Site (ECS) peptide (D-Y-K-D-D-D-D-K) conjugated to KLH using maleimide. This antibody reacts with FLAGä conjugated proteins.

Packing Type :

Ambient

Physical State :

Lyophilized

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against the FLAGä epitope tag and is useful in determining its presence in over expressed proteins in various assays. The antibody recognizes the FLAGä epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Reconstitution Buffer :

Restore with deionized water (or equivalent)

Reconstitution Volume :

100 µL

Species Reactivity :

Carboxy and amino terminal linked FLAG™ tagged recombinant proteins

Stabilizer :

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Storage :

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti DYKDDDDK DyLight™ 800 Conjugated, rabbit antibody for the detection of FLAG™ conjugated proteins DyLight™ 800 Conjugated

Background :

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.

Epitope Tag Type :

FLAG

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Application Text :

ELISA,FLISA,IF Microscopy,Western Blot,

Other :

User Optimized

Category :

GFP and Epitopes

Conjugation Name :

DyLight™ 800

Primary Image Name :

Anti-Tubulin and Anti-TNFa Antibodies - Western Blot

more info or order :

Rockland Immunochemicals product webpage