product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
V5 Epitope Tag Antibody Dylight™ 549 Conjugated
catalog :
600-442-378
quantity :
100 µg
clonality :
polyclonal
host :
domestic rabbit
conjugate :
DyLight549
application :
western blot
more info or order :
Rockland Immunochemicals product webpage
product information
Catalog Number :
600-442-378
Name :
Anti-V5 EPITOPE TAG (RABBIT) Antibody DyLight™ 549 Conjugated - 600-442-378
Display Name :
V5 Epitope Tag Antibody Dylight™ 549 Conjugated
Application Note :
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
1.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
DL549
Size :
100 µg
Default Unit :
µg
FLISA Dilution :
>1:20,000
Fluorochrome Protein Value :
3.6
IF Microscopy Dilution :
>1:5,000
Western Blot Dilution :
1:10,000 - 1:25,000
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 95-108 (G-K-P-I-P-N-P-L-L-G-L-D-S-T) of the V protein conjugated to KLH using maleimide.
Packing Type :
Ambient
Physical State :
Lyophilized
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
This affinity purified antibody is directed against the V5 epitope tag and is useful in determining its presence in over expressed proteins in various assays.  The antibody recognizes the V5 epitope tag (Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Reconstitution Buffer :
Restore with deionized water (or equivalent)
Reconstitution Volume :
100 µL
Stabilizer :
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Storage :
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Synonyms :
Rabbit Anti-V5 Epitope Tag DyLight 549™ Conjugated Antibody, Rabbit Anti V5 Epitope Tag Antibody DyLight 549™ Conjugation, Rabbit Anti-V5 Tag DyLight 549™ Conjugated Antibody
Background :
In order to improve expression levels, solubility, folding, purification and detection of recombinant proteins, a very common strategy is the fusion of peptides or proteins also known as “tags”, to the target protein. Because these tags are entities with known sequences and well characterized physicochemical properties, they are an essential tool in molecular biology that facilitates expression and purification of recombinant proteins. Because fusion tags constitute themselves antigenic epitopes for which antibodies can be developed they particularly useful for specific detection of the target protein. This Anti-V5 Epitope Tag Antibody generated in rabbit is conjugated to DyLight™549.
Epitope Tag Type :
V5
Immunogen Type :
Peptide
Low Endotoxin :
No
Sample Size :
No
Application Text :
FLISA,IF Microscopy,Western Blot,
Other :
User Optimized
Category :
GFP and Epitopes
Conjugation Name :
DyLight™ 549
Primary Image Name :
Anti-Tubulin and Anti-TNFa Antibodies - Western Blot
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.