VSV-G Epitope Tag Antibody Biotin Conjugated | Rockland Immunochemicals 600-406-386 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

VSV-G Epitope Tag Antibody Biotin Conjugated

catalog :

600-406-386

quantity :

100 µg

price :

367.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

biotin

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-406-386

Name :

Anti-VSV-G EPITOPE TAG (RABBIT) Antibody Biotin Conjugated - 600-406-386

Display Name :

VSV-G Epitope Tag Antibody Biotin Conjugated

Application Note :

This affinity purified antibody has been tested for use in ELISA and by immunofluorescence microscopy. Specific conditions for reactivity should be optimized by the end user. For standard indirect immunofluorescence assays, we recommend paraformaldehyde fixation with detergent permeabilization of cells infected with VSV or transfected with the wild-type VSV-G protein. Staining is quite clean and specific for both sources of the G protein when diluted at least 1/200. At higher concentrations, a filamentous background staining may be present.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

BAC

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000 - 1:50,000

Immunohistochemistry Dilution :

1:1,000 - 1:5,000

Western Blot Dilution :

1:2,000 - 1:10,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding amino acids near the carboxy terminal of vesicular stomatitis virus glycoprotein (VSV-G).

Packing Type :

Ambient

Physical State :

Lyophilized

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against VSV-G protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification followed by biotin conjugation, subsequent purification and exhaustive dialysis against the buffer stated above.

Reconstitution Buffer :

Restore with deionized water (or equivalent)

Reconstitution Volume :

100 µL

Stabilizer :

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Storage :

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

Rabbit Anti-VSV-G Epitope Tag Biotin Conjugated Antibody, Rabbit Anti VSV-G Epitope Tag Biotin Conjugated Antibody, Rabbit Anti-VSV-G Tag Antibody Biotin Conjugation

Background :

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. VSV-G, or vesicular stomatitis virus glycoprotein, is found within the pseudo lentiviral cloning vector pHCMV-VSV-G.

Epitope Tag Type :

VSV-G

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Other :

User Optimized

Category :

GFP and Epitopes

Conjugation Name :

Biotin

2020 Price :

367.00 USD

Primary Image Name :

Rabbit anti-VSV-G epitope tag biotin conjugated

more info or order :

Rockland Immunochemicals product webpage