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company name :
Rockland Immunochemicals
product type :
antibody
product name :
Phosphotyrosine Antibody Biotin Conjugated
catalog :
600-406-262
quantity :
100 µg
clonality :
polyclonal
host :
domestic rabbit
conjugate :
biotin
application :
western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry
more info or order :
Rockland Immunochemicals product webpage
product information
Catalog Number :
600-406-262
Name :
Anti-PhosphoTyrosine (pY) (RABBIT) Antibody Biotin Conjugated - 600-406-262
Display Name :
Phosphotyrosine Antibody Biotin Conjugated
Application Note :
Anti-PhosphoTyrosine antibody is suitable for use in ELISA and western blotting assays.  The antibody reacts specifically with phosphotyrosine  and shows minimal reactivity by ELISA and western blotting with proteins containing phosphothreonine or phosphoserine residues.  The antibody reacts with free phosphotyrosine, phosphotyrosine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysates.  Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
1.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
BAC
Size :
100 µg
Default Unit :
µg
ELISA Dilution :
1:5,000
Flow Cytometry Dilution :
Yes
Immunohistochemistry Dilution :
User Optimized
Immunoprecipitation Dilution :
User Optimized
Western Blot Dilution :
1:1,000
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-PhosphoTyrosine was prepared from whole rabbit serum produced by repeated immunizations with phosphotyrosine conjugated KLH.
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
Anti-PhosphoTyrosine was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated tyrosine peptide coupled to agarose followed by conjugation to biotin and subsequent purification.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Biotin.  Reactivity is specific for phosphotyrosine and minimal cross reactivity is observed against phosphothreonine or phosphoserine.
Species Reactivity :
phosphotyrosine
Stabilizer :
50% (v/v) Glycerol
Storage :
Store Anti-pY at -20° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.
Synonyms :
PhosphoTyrosine (pY)reactive antibody, PhosphoTyrosine (pY) polyclonal antibody, PhosphoTyrosine antibody produced in rabbit
Background :
Anti-PhosphoTyrosine detects phophorylated Tyrosines. Tyrosine is one of the 22 amino acids that are used by cells to synthesize proteins. Tyrosine has a phenol group. It functions as a receiver of phosphate groups that are transferred by way of protein kinases. Phosphorylation of the hydroxyl group changes the activity of the target protein. Anti-PhosphoTyrosine Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Immunology, Cancer research and Signal Transduction research.
Immunogen Type :
Other
Low Endotoxin :
No
Sample Size :
No
Application Text :
ELISA,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,
Other :
User Optimized
Category :
Primary Antibodies
Conjugation Name :
Biotin
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.