product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
Collagen Type I Antibody Biotin Conjugated
catalog :
600-406-103
quantity :
100
price :
522.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
biotin
reactivity :
human, mouse, rat
application :
western blot, flow cytometry, dot blot
more info or order :
Rockland Immunochemicals product webpage
citations: 13
Published Application/Species/Sample/DilutionReference
  • western blot; human; fig 2c
Sinha S, Hoshino D, Hong N, Kirkbride K, Grega Larson N, Seiki M, et al. Cortactin promotes exosome secretion by controlling branched actin dynamics. J Cell Biol. 2016;214:197-213 pubmed publisher
  • flow cytometry; mouse; fig 6
Gupta S, Itagaki R, Zheng X, Batkai S, Thum S, Ahmad F, et al. miR-21 promotes fibrosis in an acute cardiac allograft transplantation model. Cardiovasc Res. 2016;110:215-26 pubmed publisher
D Alessandro Gabazza C, Yasuma T, Kobayashi T, Toda M, Abdel Hamid A, Fujimoto H, et al. Inhibition of lung microbiota-derived proapoptotic peptides ameliorates acute exacerbation of pulmonary fibrosis. Nat Commun. 2022;13:1558 pubmed publisher
Reinhardt J, Breuer C. Fibrocytes: A Critical Review and Practical Guide. Front Immunol. 2021;12:784401 pubmed publisher
Afroj T, Mitsuhashi A, Ogino H, Saijo A, Otsuka K, Yoneda H, et al. Blockade of PD-1/PD-L1 Pathway Enhances the Antigen-Presenting Capacity of Fibrocytes. J Immunol. 2021;206:1204-1214 pubmed publisher
Oh H, Masuno K, Okusa N, Makita Y, Fujiwara S, Imamura Y. Effect of Orento, a Traditional Japanese Medicine, on IL-6, IL-8 Secretion, Type 1 Collagen Production and Alkaline Phosphatase Secretion in the Human Osteosarcoma Cell Line Saos-2. Medicines (Basel). 2020;7: pubmed publisher
Palano G, Jansson M, Backmark A, Martinsson S, Sabirsh A, Hultenby K, et al. A high-content, in vitro cardiac fibrosis assay for high-throughput, phenotypic identification of compounds with anti-fibrotic activity. J Mol Cell Cardiol. 2020;142:105-117 pubmed publisher
Kunimura K, Sakata D, Tun X, Uruno T, Ushijima M, Katakai T, et al. S100A4 Protein Is Essential for the Development of Mature Microfold Cells in Peyer's Patches. Cell Rep. 2019;29:2823-2834.e7 pubmed publisher
Ozono Y, Shide K, Toyoshima F, Takaishi Y, Tsuchimochi M, Kamiunten A, et al. Monocyte-derived fibrocytes elimination had little contribution on liver fibrosis. Hepatobiliary Pancreat Dis Int. 2019;: pubmed publisher
Heukels P, van Hulst J, van Nimwegen M, Boorsma C, Melgert B, van den Toorn L, et al. Fibrocytes are increased in lung and peripheral blood of patients with idiopathic pulmonary fibrosis. Respir Res. 2018;19:90 pubmed publisher
Collins S, Chan Li Y, Oh M, Vigeland C, Limjunyawong N, Mitzner W, et al. Vaccinia vaccine-based immunotherapy arrests and reverses established pulmonary fibrosis. JCI Insight. 2016;1:e83116 pubmed
Buyandelger B, Mansfield C, Kostin S, Choi O, Roberts A, Ware J, et al. ZBTB17 (MIZ1) Is Important for the Cardiac Stress Response and a Novel Candidate Gene for Cardiomyopathy and Heart Failure. Circ Cardiovasc Genet. 2015;8:643-52 pubmed publisher
Hashimoto N, Jin H, Liu T, Chensue S, Phan S. Bone marrow-derived progenitor cells in pulmonary fibrosis. J Clin Invest. 2004;113:243-52 pubmed
product information
Catalog Number :
600-406-103
Name :
Anti-Collagen Type I (RABBIT) Antibody Biotin Conjugated - 600-406-103
Display Name :
Collagen Type I Antibody Biotin Conjugated
Application Note :
Anti-COLLAGEN Type I Antibody Biotin Conjugated has been tested by dot blot and Flow Cytometry and is suitable for western blot, immunoprecipitation, Flow Cytometry, and immunohistochemistry. Researchers should determine optimal titers for applications that are not stated below.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
1.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
BAC
Size :
100
Default Unit :
µg
ELISA Dilution :
1:3,000 - 1:6,000
Flow Cytometry Dilution :
User Optimized
Fluorochrome Protein Value :
10-20
Immunohistochemistry Dilution :
1:50 - 1:200
Immunoprecipitation Dilution :
1:100
Western Blot Dilution :
1:3,000 - 1:6,000
Expiration :
Expiration date is one (1) year from date of receipt.
Format :
IgG
Gene Name :
COL1A1
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Collagen Type I from human and bovine placenta.
Packing Type :
Ambient
Physical State :
Lyophilized
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
This product has been prepared by immunoaffinity chromatography using immobilized antigens.  Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.  This antibody reacts with most mammalian Type I collagens and has expected cross-reactivity with Type III and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.
Reconstitution Buffer :
Restore with deionized water (or equivalent)
Reconstitution Volume :
100 µL
Species Reactivity :
Human; Mouse; Rat
Tested Applications :
Dot Blot; FC; Multiplex
Suggested Applications :
ELISA; IF; IHC; WB
Stabilizer :
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Storage :
Store vial at 4° C prior to restoration.   Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Synonyms :
rabbit anti-collagen type I antibody biotin conjugation, biotin conjugated rabbit anti-collagen type I antibody, Collagen Of Skin Tendon And Bone, Collagen Type 1 antibody, Collagen type I alpha 1 antibody, Collagen alpha-1 (I) chain, Alpha-1 type I collagen, type 1 procollagen alpha 1
Target Species :
Mammalian
Background :
COLLAGEN Type I Antibody Biotin Conjugated is specific for Collagen Type I. Collagen Type I (Type-I collagen) is the most abundant collagen of the human body. It is present in scar tissue, the end product when tissue heals by repair. It is also found in tendons, the endomysium of myofibrils and the organic part of bone. Anti-collagen Type I antibody is suitable for Cancer research and other general research.
Immunogen Type :
Native Protein
Low Endotoxin :
No
Sample Size :
No
Application Text :
ELISA,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,
Other :
User Optimized
Category :
Primary Antibodies
Conjugation Name :
Biotin
2025 Price :
522.00 USD
UniProt :
P02452
NCBI :
NP_000079.2
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.