KLH Antibody Peroxidase Conjugated | Rockland Immunochemicals 600-403-466 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

KLH Antibody Peroxidase Conjugated

catalog :

600-403-466

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

HRP

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-403-466

Name :

Anti-KLH (RABBIT) Antibody Peroxidase Conjugated - 600-403-466

Display Name :

KLH Antibody Peroxidase Conjugated

Application Note :

Anti-KLH Antibody Peroxidase Conjugated antibody has been assayed against 1.0 ug of KLH in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:60,000 is suggested for this product. Anti-KLH Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Peroxidase conjugated anti-KLH antibody is suitable for use with streptavidin reporter systems. Researchers should determine optimal titers for applications that are not stated below.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

HRP

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:400,000

Immunohistochemistry Dilution :

1:500 - 1:2,500

Western Blot Dilution :

1:1,000 - 1:5,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

KLH (Keyhole Limpet Hemocyanin

Packing Type :

Ambient

Physical State :

Lyophilized

Preservative :

0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Purity and Specificity :

Anti-KLH (RABBIT) Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using KLH coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, and KLH.

Reconstitution Buffer :

Restore with deionized water (or equivalent)

Reconstitution Volume :

100 µL

Stabilizer :

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Storage :

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit Anti-KLH antibody peroxidase conjugation, HRP conjugated rabbit Anti-KLH antibody, Keyhole Limpet Hemocyanin

Background :

KLH Antibody, Peroxidase Conjugated, detects Keyhole Limpet Hemocyanin protein which is a very large, heterogeneous glycosylated protein consisting of subunits with a molecular weight of 350,000 and 390,000. KLH readily forms aggregates with molecular weights of 4,500,000-13,000,000 daltons. Each domain of a KLH subunit contains two copper atoms that together bind a single oxygen molecule (O2). When oxygen is bound to hemocyanin, the molecule takes on a distinctive transparent, opalescent blue color. The KLH protein is potently immunogenic yet safe in humans and is therefore highly prized as a vaccine carrier protein. The large and highly glycosylated KLH protein cannot be reproduced synthetically. It is available only as a purified biological product from the Keyhole Limpet Megathura crenulata. KLH is being tested in a variety of cancer vaccines, including non-Hodgkins lymphoma, cutaneous melanoma, breast and bladder cancer.

Immunogen Type :

Native Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Other :

User Optimized

Category :

GFP and Epitopes

Conjugation Name :

Peroxidase (Horseradish)

Primary Image Name :

Anti KLH Polyclonal Antibody - Western blot

more info or order :

Rockland Immunochemicals product webpage