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company name :
Rockland Immunochemicals
product type :
antibody
product name :
BUB1 Antibody
catalog :
600-401-Z98
quantity :
100 µg
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, ELISA
more info or order :
Rockland Immunochemicals product webpage
product information
Catalog Number :
600-401-Z98
Name :
Anti-BUB1 (RABBIT) Antibody - 600-401-Z98
Display Name :
BUB1 Antibody
Application Note :
Anti-Bub1 Antibody has been tested for use in ELISA, Western Blotting, Immunocytochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 122 kDa in Western Blots of specific cell lysates and tissues.
Buffer :
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
1 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
(None)
Size :
100 µg
Default Unit :
µg
ELISA Dilution :
1:20,000-1:40,000
IF Microscopy Dilution :
20 μg/mL
Western Blot Dilution :
2-4 μg/mL
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG
Gene Name :
BUB1
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-Bub1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 19 amino acid synthetic peptide from near the N-terminus of human Bub1.
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.02% (w/v) Sodium Azide
Purity and Specificity :
Anti-Bub1 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with Bub1 from other sources has not been determined.
Species Reactivity :
Human, mouse
Storage :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Synonyms :
Bub1 Antibody, BUB1A, BUB1L, hBUB1, BUB1A
Target Species :
Human
Background :
Bub1 was initially identified as the mammalian homolog to the yeast protein through a cDNA screen. Bub1 encodes a kinase involved in the formation of the spindle checkpoint, an important mechanism that ensures high fidelity mitotic chromosome segregation. It is thought to be required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshim protein and metaphase congression. Bub1 functions by phosphorylating cdc20, a member of the mitotic checkpoint complex and activating the spindle checkpoint. A related protein kinase Bub3 interacts with Bub1 and targets it to kinetochores prior to chromosome alignment. Mutations in bub1 have been associated with aneuploidy and several forms of cancer.
Immunogen Type :
Peptide
Low Endotoxin :
No
Sample Size :
No
Target Domain of Reactivity :
N-Terminus
Application Text :
ELISA,IF Microscopy,Western Blot,
Other :
ICC:10 μg/mL
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
UniProt :
O43683
NCBI :
O43683
Primary Image Name :
Immunocytochemistry of Bub1 Antibody
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.