MBD1 Antibody | Rockland Immunochemicals 600-401-X42 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

MBD1 Antibody

catalog :

600-401-X42

quantity :

50 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, chromatin immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-X42

Name :

Anti-MBD1 (RABBIT) Antibody - 600-401-X42

Display Name :

MBD1 Antibody

Application Note :

Anti-MBD1 Antibody is suitable for Chromatin Immunoprecipitation, ELISA and Western Blots. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 75 kDa in the appropriate cell lysate or extract.

Buffer :

0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

0.7 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

50 µg

Default Unit :

µg

ELISA Dilution :

1:1,000

ChIP Dilution :

1.5 μg per IP

Western Blot Dilution :

1:500

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

MBD1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-MBD1 Antibody was produced in rabbits by repeated immunizations with a synthetic peptide containing a sequence from the N-terminus of human MBD1.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.05% (w/v) Sodium Azide and 0.05% ProClin 300

Purity and Specificity :

Anti-MBD1 Antibody was purified by affinity purification. Cross reactivity with other species was not tested.

Species Reactivity :

Human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG

Synonyms :

Methyl-CpG-binding domain protein 1, CXXC-type zinc finger protein 3, Methyl-CpG-binding protein MBD1, Protein containing methyl-CpG-binding domain 1, CXXC3, PCM1

Target Species :

Human

Background :

MBD1 is a transcriptional repressor that specifically binds to methylated CpG dinucleotides in promoter sequences. MBD1 acts by recruiting a variety of histone deacetylases (HDAC’s) and chromatin remodelling factors. MBD1-dependent transcriptional repression is mediated by ATF7IP through the recruitment of factors such as the histone methyltransferase SETDB1. MBD1 probably forms a complex with SETDB1 and ATF7IP which couples DNA methylation to H3K9 trimethylation and represses transcription. Anti-MBD1 Antibody is ideal for research in Epigenetics, Gene Expression and Chromatin Remodeling.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

N-Terminus

Application Text :

ELISA,Chip,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q9UIS9

NCBI :

NP_001191065.1

Primary Image Name :

Chromatin Immunoprecipitation anti-MBD1 antibody

more info or order :

Rockland Immunochemicals product webpage