PTPH1 Phospho S459 Antibody | Rockland Immunochemicals 600-401-X22 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

PTPH1 Phospho S459 Antibody

catalog :

600-401-X22

quantity :

100 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-X22

Name :

Anti-PTPH1 pS459 (RABBIT) Antibody - 600-401-X22

Display Name :

PTPH1 Phospho S459 Antibody

Application Note :

Anti-Phospho-Ser459 PTPH1 Antibody is suitable for Western Blots and is specific for the ~104 kDa PTPH1 protein phosphorylated at Ser459. Immunolabeling is blocked by preadsorption of the antibody with the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is also completely eliminated by treatment with lambda-phosphatase. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Clonality :

Polyclonal

Conjugation :

(None)

Size :

100 µL

Default Unit :

ELISA Dilution :

1:10,000

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

PTPN3

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-Phospho-Ser459 PTPH1 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding the phospho-Ser459 of human PTPH1 conjugated to KLH.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Purity and Specificity :

Anti-Phospho-Ser459 PTPH1 Antibody is directed against PTPH1 protein phosphorylated at S459. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated PTPH1. Minimal reactivity occurs against non-phosphorylated PTPH1. Reactivity against PTPH1 occurs from human sources. However, reactivity is also expected against bovine, canine, non-human primate, and sheep based on 100% sequence homology.

Species Reactivity :

human, mouse

Stabilizer :

0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol

Storage :

Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilut

Synonyms :

PTPN3, Tyrosine-protein phosphatase non-receptor type 3, Protein-tyrosine phosphatase H1, PTP-H1, PTPH1

Target Species :

Human

Background :

Protein-tyrosine Phosphatase H1 (PTPH1) has recently been identified as a specific p38γ MAPK phosphatase which is mediated through PDZ interaction. Ras has been demonstrated to increase both p38 gamma and PTPH1 protein expression, and there is a coupling of increased p38 gamma and PTPH1 protein expression in primary colon cancer tissues. Phosphorylation of PTPH1 at Ser459 leads to PTPH1 stabilization, which plays an important role in Ras oncogenesis and stress response. Additionally, phosphorylation of PTPH1 Ser459 reveals a novel mechanism by which MAPK signals through PTPH1 to regulate cellular response. PTPH1 pS459 Antibody is ideal for researchers interested in cancer research.

Immunogen Type :

Peptide

Low Endotoxin :

Post Translational Modification :

Phosphorylation

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P26045

NCBI :

NP_002820.3

more info or order :

Rockland Immunochemicals product webpage