PAK 1 Phospho T84 Antibody | Rockland Immunochemicals 600-401-X21 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

PAK 1 Phospho T84 Antibody

catalog :

600-401-X21

quantity :

100 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-X21

Name :

Anti-PAK-1 pT84 (RABBIT) Antibody - 600-401-X21

Display Name :

PAK 1 Phospho T84 Antibody

Application Note :

Anti-Phospho-Thr84 PAK1 Antibody is suitable for Western Blots and is specific for the ~68 kDa PAK1 phosphorylated at Thr84. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Clonality :

Polyclonal

Conjugation :

(None)

Size :

100 µL

Default Unit :

ELISA Dilution :

1:10,000

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

PAK1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-Phospho-Thr84 PAK1 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding the phospho-Thr84 of human PAK1 conjugated to KLH.

Packing Type :

Wet Ice

Physical State :

Liquid (sterile filtered)

Purity and Specificity :

Anti-Phospho-Thr84 PAK1 Antibody is directed against PAK1 protein phosphorylated at T84. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated PAK1. Minimal reactivity occurs against non-phosphorylated PAK1. Reactivity against PAK1 occurs from human sources. However, reactivity is also expected against bovine, canine, chicken, non-human primates, sheep, and zebra fish based on 100% sequence homology.

Species Reactivity :

human, mouse, rat

Stabilizer :

0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol

Storage :

Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilut

Synonyms :

Serine/Threonine-protein kinase PAK 1, Alpha-PAK, p21-activated kinase 1, p65-PAK

Target Species :

Human

Background :

PAKs, p21 activated kinases, are a family of serine/threonine protein kinases comprised of six isoforms, PAK1-6, and they play important roles in cytoskeleton dynamics, cell survival and proliferation. Each of these isoforms contains a C-terminal catalytic domain and an N-terminal regulatory domain with a small G protein binding motif. OSR1, oxidative stress response 1, is activated only by osmotic stresses, like sorbital or NaCl. It has been predicted that OSR1 phosphorylates PAK1 in the regulatory domain at thr84 and inhibits activation of JNK and MAPK pathway. It has also been suggested that OSR1 may have a regulating function with actin cytoskeleton because it can phosphorylate PAK1 at thr84 and bind to gelsolin. PAK1 pT84 Antibody is ideal for researchers interested in cell cycle research.

Immunogen Type :

Peptide

Low Endotoxin :

Post Translational Modification :

Phosphorylation

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q13153

NCBI :

NP_001122092.1

more info or order :

Rockland Immunochemicals product webpage