Histone H2A.Zac Antibody | Rockland Immunochemicals 600-401-V36 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Histone H2A.Zac Antibody

catalog :

600-401-V36

quantity :

50 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, chromatin immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-V36

Name :

Anti-Histone H2 A.Zac (RABBBIT) Antibody - 600-401-V36

Display Name :

Histone H2A.Zac Antibody

Application Note :

Anti-H2A.Zac Antibody is suitable for Chromatin Immunoprecipitation, Dot Blotting, ELISA, Immunofluorescence and Western Blots. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15 kDa in the appropriate cell lysate or extract.

Buffer :

0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.09 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

50 µg

Default Unit :

µg

ELISA Dilution :

1:500

ChIP Dilution :

1 μg/ChIP

IF Microscopy Dilution :

1:500

Western Blot Dilution :

1:1,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

H2AFZ

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-H2A.Zac Antibody was produced in rabbits by repeated immunization with a synthetic peptide containing the region of human histone H2A.Z acetylated at lysines 5, 7 and 11.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.05% (w/v) Sodium Azide and 0.05% ProClin 300

Purity and Specificity :

Anti-H2A.Zac Antibody was purified by affinity purification. Cross-reactivity with Histone 2A.Z acetylated is expected amongst a wide range of species due to homology.

Species Reactivity :

Human, Mouse, wide range expected

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG

Synonyms :

Histone H2A.Z, H2A/z

Target Species :

Human

Background :

Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of the histone H2A variant H2A.Z is associated with the promoters of active genes. Anti-H2A.Zac Antibody is ideal for research in Chromatin Remodeling and Epigenetics.

Immunogen Type :

Peptide

Low Endotoxin :

Post Translational Modification :

Acetylation

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Chip,IF Microscopy,Western Blot,

Other :

Dot Blot: 20,000

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P0C0S5

NCBI :

NP_002097

Primary Image Name :

Immunofluorescence Microscopy of anti-Histone H2 A.Zac antibody

more info or order :

Rockland Immunochemicals product webpage