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company name :
Rockland Immunochemicals
product type :
antibody
product name :
Anti-mCherry Antibody Pre-Adsorbed
catalog :
600-401-P16
quantity :
100
price :
335.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
application :
western blot, ELISA
more info or order :
citations: 22
| Reference |
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Kao R, Rurik J, Farr G, Dong X, Majesky M, Maves L. Pbx4 is Required for the Temporal Onset of Zebrafish Myocardial Differentiation. J Dev Biol. 2015;3:93-111 pubmed
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product information
Catalog Number :
600-401-P16
Name :
Anti-mCherry (RABBIT) Antibody (Min X Hu Ms & Rt Serum Proteins) - 600-401-P16
Display Name :
Anti-mCherry Antibody Pre-Adsorbed
Application Note :
Polyclonal anti-mCherry is designed to detect mCherry, RFP, and its variants. Anti-mCherry (Discosoma sp.) has been tested by ELISA and Western blot and is intended for use in immunological assays including ELISA, western blotting, immunofluorescence, and fluorescence activated cell sorting (FACS). Researchers should determine optimal titers for applications that are not stated. In addition, we performed conjugation of RFP antibodies to either fluorescent dyes, biotin or horseradish peroxidase to further facilitate RFP protein detection and quantification.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
1.17
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
(None)
Size :
100
Default Unit :
µg
ELISA Dilution :
1:150,000 - 1:250,000
Immunohistochemistry Dilution :
1:200 - 1:2,000
IF Microscopy Dilution :
1:200 - 1:2,000
Western Blot Dilution :
1:2,000 - 1:10,000
Expiration :
Expiration date is one (1) year from date of receipt.
Format :
IgG
Gene Name :
DsRed
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
The immunogen is a mCherry mutant variant fusion protein of RFP corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
mCherry was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Expect reactivity against mCherry, RFP and its variants: tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified mCherry. No reaction was observed against Human, Mouse or Rat serum proteins. ELISA was used to confirm specificity at less than 0.1% of target signal.
Species Reactivity :
RFP; mCherry; rRFP
Tested Applications :
ELISA; WB
Suggested Applications :
IF; IHC; IP; Multiplex
Storage :
Store mCherry Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Synonyms :
rabbit anti-mCherry antibody, RFP, mCherry monomeric red fluorescent protein, Red Fluorescent Protein (RFP), rDsRed, Discosoma sp. Red Fluorescent Protein, mRFP1, mBanana, mHoneydew, mPlum, mOrange, mStrawberry, mTangerine
Background :
mCherry Antibody is ideal for Western Blotting. Fluorescent proteins such as Discosoma Red Fluorescent Protein (and its variants), and GFP are widely used in research practice. Both commonly serve as a markers for gene expression and protein localization. DsRed was isolated from sea anemone Discosoma sp. mushroom and GFP is originated from Aequorea victoria jellyfish. As DsRed and GFP share only 19% identity, therefore, in general, anti-GFP antibodies do not recognize DsRed protein and vice versa. Structurally, Discosoma red fluorescent protein is similar to Aequorea green fluorescent protein in terms of its overall fold (a β-can) and chromophore-formation chemistry. However, Discosoma red fluorescent protein undergoes an additional steps in the chromophore maturation and obligates tetrameric structure. All mCherry antibodies have been pre-absorbed to eliminate any potential cross-reactivity to human, mouse and rat serum proteins. The antibodies are also confirmed for non-reactivity to GFP protein.
Low Endotoxin :
No
Sample Size :
No
Application Text :
ELISA,Immunohistochemistry,IF Microscopy,Western Blot,
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
2025 Price :
335.00 USD
more info or order :
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.
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