Histone H3 K79-Me1 Antibody | Rockland Immunochemicals 600-401-P10 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Histone H3 K79-Me1 Antibody

catalog :

600-401-P10

quantity :

50 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, chromatin immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-P10

Name :

Anti-Histone H3 [Monomethyl Lys79] (RABBIT) Antibody - 600-401-P10

Display Name :

Histone H3 K79-Me1 Antibody

Application Note :

Anti-Histone H3 K79-Me1 antibody is useful in Western Blot, ChIP, Immunofluorescence, and Dot Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15kDa corresponding to the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

0.50 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

50 µg

Default Unit :

µg

ChIP Dilution :

2 µg

IF Microscopy Dilution :

1:50 - 1:100

Western Blot Dilution :

0.5µg/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

HIST1H3A

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Histone H3 K79-Me1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide surrounding the Lys79 site of human Histone H3.

Other Performance Data :

Dot Blot - 1:1000

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-Histone H3 [Monomethyl Lys79] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

Species Reactivity :

Human, mouse, monkey

Stabilizer :

30% Glycerol

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-Histone H3 monomethyl Lys79 antibody, H3K79me1, Anti-H3 K79-Me1, Histone H3 antibodies

Target Species :

Human

Background :

Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular Lys79 methylations are involved in transcriptional activation, and has been found to be inversely correlated with H2B ubiquitination. Anti-Histone H3 K79-Me1 antibody is ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.

Immunogen Type :

Peptide

Low Endotoxin :

Post Translational Modification :

Methylation

Sample Size :

Application Text :

Chip,IF Microscopy,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P84243

NCBI :

NP_002098.1

Primary Image Name :

Histone H3 K79me1 Immunofluorescence

more info or order :

Rockland Immunochemicals product webpage