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company name :
Rockland Immunochemicals
product type :
antibody
product name :
Histone H3 K9-Ac/pS10 (RABBIT) Antibody
catalog :
600-401-P08
quantity :
50 µg
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot
more info or order :
Rockland Immunochemicals product webpage
product information
Catalog Number :
600-401-P08
Name :
Anti-Histone H3 [ac Lys9/phospho Ser10] (RABBIT) Antibody - 600-401-P08
Display Name :
Histone H3 K9-Ac/pS10 (RABBIT) Antibody
Application Note :
Anti-Histone H3 K9-Ac/pS10 antibody is useful for Western Blot, Dot Blot, and Immunocytochemistry/Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15 kDa corresponding to Histone H3 protein by Western Blotting in NIH-3T3 histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
0.25 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
(None)
Size :
50 µg
Default Unit :
µg
IF Microscopy Dilution :
1:10
Western Blot Dilution :
1.0 µg/mL
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG
Gene Name :
HIST2H3C
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-Histone H3 [ac Lys9/phospho Ser10] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide with a acetylation surrounding Lysine 9 and a phosphorylation surrounding serine 10 of human Histone H3.
Other Performance Data :
Dot Blot: 1:1000
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
Anti-Histone H3 K9Ac/pS10 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3. Cross-reactivity with other sources has not been determined.
Species of Origin :
Rabbit
Species Reactivity :
Human, Mouse
Storage :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Synonyms :
rabbit anti-Histone H3 Ac Lys9 pS10 antibody, Histone H3 antibodies, H3K9ac/pS10
Target Species :
Human
Background :
Histones of the nucleosome build chromatin and undergo various post-translational modifications proven to regulate chromatin condensation and DNA accessibility. Phosphorylations on Serine 10 has been shown to facilitate chromatin condensation by its interaction with Aurora-B kinase during mitosis and is alternatively active in cell division. Serine modification is associated with IKK-alpha and Snf1 enzymes in transcriptional activation, and is modified by MSK1 and 2 which are enzymes that react to growth factors and cellular stress in immediate-early gene activation. Lysine 9 acetylations are connected with histone deposition and transcriptional activation. Anti-Histone H3 [ac Lys9/phospho Ser10] antibody is ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.
Immunogen Type :
Peptide
Low Endotoxin :
No
Post Translational Modification :
Dual Modification
Sample Size :
No
Application Text :
IF Microscopy,Western Blot,
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
UniProt :
Q71DI3
NCBI :
NP_001005464
Primary Image Name :
Anti-Histone H3 K9Ac/pS10-IF
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.