Histone H4 K12-Ac Antibody | Rockland Immunochemicals 600-401-P06 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Histone H4 K12-Ac Antibody

catalog :

600-401-P06

quantity :

50 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-P06

Name :

Anti-Histone H4 [ac Lys12] (RABBIT) Antibody - 600-401-P06

Display Name :

Histone H4 K12-Ac Antibody

Application Note :

Anti-Histone H4 K12 ac antibody is useful for Western Blot, Dot Blot, and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~11 kDa corresponding to Histone H4 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

0.69 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

50 µg

Default Unit :

µg

Immunohistochemistry Dilution :

1:100

IF Microscopy Dilution :

1:10

Western Blot Dilution :

1.0 ug/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

HIST4H4

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Histone H4 [ac Lys12] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 12 of human Histone H4.

Other Performance Data :

Dot Blot: 1:1000

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-Histone H4 [ac Lys12] was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Alfalfa based on 100% sequence homology. Cross-reactivity with Histone H4 [ac Lys12] from other sources has not been determined.

Species Reactivity :

Human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-Histone H4 Ac Lys12 antibody, H4K12ac, HIST2H4B, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, histone cluster 4, H4, histone 4, H4

Target Species :

Human

Background :

Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, acetylation of H4 Lys5 (H4 Lys5Ac) has been linked to transcriptional activation and DNA repair. Newly assembled histones are typically acetylated on H4 at lysine 5 and 12. The enzyme histone acetyltransferase 1 (HAT1) is the primary modulator, and these marks are necessary for complete chromatin assembly. Research suggests that [ac Lys12] is associated with memory repair and telomere replication. Anti-Histone H4 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.

Immunogen Type :

Peptide

Low Endotoxin :

Post Translational Modification :

Acetylation

Sample Size :

Application Text :

Immunohistochemistry,IF Microscopy,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P62805

NCBI :

NP_001029249

Primary Image Name :

600-401-P06-Anti-HistoneH4-K12-Ac if/icc

more info or order :

Rockland Immunochemicals product webpage