product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
Anti-Bacteriophage M13 Antibody
catalog :
600-401-MR5
quantity :
100 µg
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
application :
western blot, ELISA
more info or order :
image
image 1 :
ELISA results of purified polyclonal Rabbit Bacteriophage M13 Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5μg/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Coating Buffer pH9.5, Goat Anti-Rabbit HRP conjugated (p/n 611-103-122) at 1:30,000 and substrate (p/n TMBE-1000).
product information
Catalog Number :
600-401-MR5
Name :
Anti-Bacteriophage M13 (RABBIT) Antibody - 600-401-MR5
Display Name :
Anti-Bacteriophage M13 Antibody
Application Note :
Anti-Bacteriophage M13 Antibody has been tested in ELISA. Expect a band at ~3.6 kDa in western blot using appropriate lysates or tissues.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
1.0 mg/ml
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
(None)
Size :
100 µg
Default Unit :
µg
ELISA Dilution :
1:10,000 - 1:50,000
IF Microscopy Dilution :
User Optimized
Western Blot Dilution :
User Optimized
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG
Gene Name :
VII
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-M13 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-terminal portion of Bacteriophage M13 conjugated to Keyhole Limpet Hemocyanin (KLH).
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
This affinity purified antibody is directed against Tail virion protein G7P. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence with Enterobacteria phage M13, Enterobacteria phage f1, and Enterobacteria phage fd.
Storage :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Synonyms :
Tail virion protein G7P, Enterobacteria phage M13, Bacteriophage M13,Coat protein C polypeptide I, Gene 7 protein
Target Species :
Human
Background :
Tail virion protein G7P (Bacteriophage M13) may initiate with G9P the virion concomitant assembly-budding process, by interacting with the packaging signal of the viral genome. The assembly-budding takes place at the host inner membrane. In turn, G7P and G9P are present at the end of the filamentous virion that emerges first from the bacterial host. Anti-Bacteriophage M13 Antibody is useful for researchers interested in HCP, Host Cell Membranes, Transmembrane Viruses, and E. coli bacteria.
Immunogen Type :
Peptide
Low Endotoxin :
No
Sample Size :
No
Target Domain of Reactivity :
N-Terminus
Application Text :
ELISA,IF Microscopy,Western Blot,
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
Primary Image Name :
ELISA of Rabbit Anti-Bacteriophage M13 Antibody
New Product :
Yes
more info or order :
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.