CREBBP Antibody | Rockland Immunochemicals 600-401-MQ0S product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

CREBBP Antibody

catalog :

600-401-MQ0S

quantity :

25 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

Western Blot of Rabbit Anti-CREBBP Antibody. Lane 1: Opal Pre-Stained Marker (p/n MB-210-0500). Lane 2: Mouse Brain Whole Tissue Lysate (p/n W10-000-T004) [+]. Lane 3: Human Eye Tissue Lysate [-]. Load: 35µg lysate/lane on a 7.5% gel. Primary Antibody: Anti-CREBBP at 5µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:70,000 at RT. Block: Universal Buffer BlockOut (p/n MB-073). Predicted MW: ~265, 494kDa (Mouse), ~265kDa (Human). Observed MW: ~260, 270kDa. Exposure: 60sec.

product information

Catalog Number :

600-401-MQ0S

Name :

Anti-CREBBP (RABBIT) Antibody - 600-401-MQ0S

Display Name :

CREBBP Antibody

Application Note :

Anti-CREBBP Antibody has been tested in ELISA and WB. Expect a band ~265kDa in western blot using appropriate tissue or lysates. Positive control used: Mouse brain, MOLT-4, A549, and PC3 lysates in Western Blots.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

25 µL

Default Unit :

ELISA Dilution :

1:10,000-1:50,000

Western Blot Dilution :

5µg/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

CREBBP

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-CREBBP antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an N-Terminal portion of human CREBBP conjugated to Keyhole Limpet Hemocyanin (KLH).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against human CREBBP. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to mouse and rat.

Species Reactivity :

Human, Mouse

Storage :

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Synonyms :

Rabbit Anti-CREBBP Antibody, Rabbit Anti-CREB Binding Protein, CREB-Binding Protein, EC 2.3.1.48, CBP, Rubinstein-Taybi Syndrome, KAT3A, MKHK1, RSTS1, RSTS

Target Species :

Human

Background :

CREBBP (CREB Binding Protein) is ubiquitously expressed. It acetylates histones, giving a specific tag for transcriptional activation and acetylates non-histone proteins, like NCOA3 and FOXO1. It binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes, so it’s known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. CREBBP acts as a coactivator of ALX1 and acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers. It acetylates PCNA, in which this acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER). Functions as a transcriptional coactivator for SMAD4 in the TGF-beta signaling pathway. This protein shares regions of very high sequence similarity with protein p300 in its bromodomain, cysteine-histidine-rich regions, and histone acetyltransferase domain. Bromodomains (BRDs) are epigenetic reader domains that selectively recognize acetylated lysine residues on the tails of histone proteins and are the only known protein modules that can target acetylated lysine residues. Mutations in this gene cause Rubinstein-Taybi syndrome (RTS) and acute myeloid leukemia. Anti-CREBBP Antibody is useful for researchers interested in Transcription Factors, Histones, Epigenetics, and Cancer research.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Yes

Target Domain of Reactivity :

N-Terminus

Application Text :

ELISA,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q92793

NCBI :

1387

Primary Image Name :

Western Blot of Rabbit Anti-CREBBP Antibody

New Product :

Yes

more info or order :

Rockland Immunochemicals product webpage