Scp3 Antibody | Rockland Immunochemicals 600-401-MP9S product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Scp3 Antibody

catalog :

600-401-MP9S

quantity :

25 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

mouse

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

Immunohistochemistry of Rabbit Anti-Scp3 Antibody. Tissue: Mouse Testis. Antigen Retrieval: HIER using Citrate buffer for 20 min. Fixative: none. Primary Antibody: Anti-Scp3 at 1:100 for 30min at RT. Secondary Antibody: Anti-Rabbit Poly-HRP-IgG Ready to Use for 8 min at RT. CounterStain: Hematoxylin. Substrate: DAB. Analysis Results: Scp3 showed weak to moderate nuclear staining of spermatocytes. Other germ cells did not stain. The specificity of the nuclear staining is consistent with Scp3 staining.

product information

Catalog Number :

600-401-MP9S

Name :

Anti-Scp3 (RABBIT) Antibody - 600-401-MP9S

Display Name :

Scp3 Antibody

Application Note :

Anti-Scp3 Antibody has been tested in ELISA, WB, and IHC. Positive control used: Mouse Testis Lysate (p/n W10-000-GZ2) in Western Blot; Mouse Testis in Immunohistochemistry.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

25 µL

Default Unit :

ELISA Dilution :

1:10,000-1:50,000

Immunohistochemistry Dilution :

1:100

Western Blot Dilution :

5µg/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

Sycp3

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-Scp3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-terminal region of mouse Scp3 conjugated to Keyhole Limpet Hemocyanin (KLH).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against mouse Scp3. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to mouse and golden hamster and 93% to rat.

Storage :

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Synonyms :

Rabbit Anti-Synaptonemal complex protein 3 Antibody, SCP-3, Sycp3, Scp3, Synaptonemal complex protein 3

Target Species :

Mouse

Background :

Synaptonemal complex protein 3 (Scp3) is a component of the synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. These proteins are required for centromere pairing during meiosis in male germ cells. It is required for normal meiosis during spermatogenesis and male fertility but plays a lesser role in female fertility. Required for efficient phosphorylation of HORMAD1 and HORMAD2. Mutations in this gene are associated with azoospermia in males and susceptibility to pregnancy loss in females. Anti-Scp3 Antibody is useful for researchers interested in Meiosis, Mitotic Cell Cycle, and fertility.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Yes

Target Domain of Reactivity :

N-Terminus

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P70281

NCBI :

NP_035647.2

Primary Image Name :

Immunohistochemistry of Rabbit Anti-Scp3 Antibody

New Product :

Yes

more info or order :

Rockland Immunochemicals product webpage