p130 Antibody | Rockland Immunochemicals 600-401-MP1 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

p130 Antibody

catalog :

600-401-MP1

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

Western Blot of Rabbit Anti-p130 Antibody. Lane 1: MOLT-4 Lysate (p/n W09-001-GK2) (1µg) [+]. Lane 2: MOLT-4 Lysate (p/n W09-001-GK2) (10µg) [+]. Lane 3: Human Eye Lysate (1µg) [-]. Lane 4: Human Eye Lysate (10µg) [-]. Lane 5: Opal Pre-stained Molecular Weight Marker (p/n MB-210-0500). Primary Antibody: Anti-p130 at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:10,000 for 1hr at RT. Blocking Buffer: Universal BlockOut (p/n MB-073). Predicted MW: ~128.4kDa. Observed MW: ~125kDa. Exposure: 15sec.

product information

Catalog Number :

600-401-MP1

Name :

Anti-p130 (RABBIT) Antibody - 600-401-MP1

Display Name :

p130 Antibody

Application Note :

Anti-p130 Antibody has been tested in ELISA and WB. Expect bands ~128.4 kDa in western blot using appropriate tissues or lysates. Positive control used: MOLT-4 lysate in western blot.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

0.92 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000 - 1:50,000

Western Blot Dilution :

1 µg/ml

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

RBL2

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-p130 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal portion of human Retinoblastoma-like protein 2 conjugated to Keyhole Limpet Hemocyanin (KLH).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against human Retinoblastoma-like protein 2. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to rat, human, and mouse.

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

Rabbit Anti-p130 Antibody, Rabbit Anti-Retinoblastoma-like protein 2 Antibody, RB Transcriptional Corepressor Like 2, 130 KDa Retinoblastoma-Associated Protein, Retinoblastoma-Related Protein 2, Retinoblastoma-Like Protein 2, Retinoblastoma-Like 2, RBR-2

Target Species :

Human

Background :

Retinoblastoma-Associated Protein (130 KDa) is a key regulator of entry into cell division. p130 is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. It recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression and controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters and is a potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. It binds to cyclins A and E, as well as, binds to and may be involved in the transforming capacity of the adenovirus E1A protein. p130 may act as a tumor suppressor. Anti-p130 Antibody may be useful for researchers interested in Adipogenesis, Retinoblastoma, and Retinal Cancer.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

ELISA,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q08999

NCBI :

NP_001310537.1

Primary Image Name :

Western Blot of Rabbit Anti-p130 Antibody

New Product :

Yes

more info or order :

Rockland Immunochemicals product webpage