Smad1 Antibody | Rockland Immunochemicals 600-401-MN6 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Smad1 Antibody

catalog :

600-401-MN6

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

mouse

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

Immunofluorescence of Rabbit Anti-Smad1 Antibody. Cells: C2C12 cells. Fixative: 100% MeOH. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-Smad1 at 5µg/mL overnight at 2-8°C. Secondary Antibody: Donkey Anti-Rabbit IgG DyLight™488 (p/n 611-741-127) at 5µg/mL for 1hr at RT. Nurclear Counterstain: DAPI. Staining: (A) DAPI. (B) Anti-Smad1 + DyLight™488. (C) Merge A+B. (D) Secondary Only. Expected localization: Nucleus, Plasma membrane, and Cytosol.

product information

Catalog Number :

600-401-MN6

Name :

Anti-Smad1 (RABBIT) Antibody - 600-401-MN6

Display Name :

Smad1 Antibody

Application Note :

Anti-Smad1 Antibody has been tested in ELISA, WB, IF, and IHC. Expect a band at ~52.2kDa in western blot using appropriate tissues or lysates. Positive control used: Mouse testis and C2C12 in Western Blot; C2C12 in Immunofluorescence; Mouse Lung, Skeletal Muscle, and Testis in Immunohistochemistry.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

5µg/mL

Immunohistochemistry Dilution :

1:400-1:500

IF Microscopy Dilution :

5-10µg/mL

Western Blot Dilution :

5µg/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

Smad1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-Smad1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of mouse Smad1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against mouse Smad1. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence and 83% homology to human and rat.

Species Reactivity :

Mouse

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

Mothers against decapentaplegic homolog 1, MAD homolog 1, Mothers against DPP homolog 1, Dwarfin-A, Dwf-A, Mothers-against-DPP-related 1, Mad-related protein 1, mMad1, SMAD 1, Smad1, Madh1, Madr1

Target Species :

Mouse

Background :

Smad1 is also known Mothers Against Decapentaplegic homolog 1, Mothers against DPP homolog 1, hSMAD-3, JV4-1, Transforming growth factor-Beta-Signaling Protein 1 or BSP1. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD1, as a transcriptional modulator, is activated by BMP (Bone Morphogenetic Protein) type 1 receptor kinase (it is a receptor-regulated SMAD or R-SMAD). BMPs are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. SMAD proteins have been implicated as downstream effectors of TGF beta/BMP signaling. In response to BMP ligands, SMAD1 can be phosphorylated (other sites besides the most prominent of S206, are S187, S195, and S214). S-206 is phosphorylated by ERK in response to mitogenic growth factors, or by recombinant ERK in vitro; this can be tested by treating cells with EGF or in cancer cells where Ras is activated. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is also a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Immunohistochemistry,IF Microscopy,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P70340

NCBI :

NP_032565.2

Primary Image Name :

Immunofluorescence of Rabbit Anti-Smad1 Antibody

New Product :

Yes

more info or order :

Rockland Immunochemicals product webpage