Cxcl1 Antibody | Rockland Immunochemicals 600-401-MN2 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Cxcl1 Antibody

catalog :

600-401-MN2

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

Immunohistochemistry of Rabbit Anti-Cxcl1 Antibody. Tissue: Mouse Lung. Fixative: None. Antigen Retrieval: HIER using Citrate Buffer for 20 mins. Primary Antibody: Anti-Cxcl1 at 1:100 for 30 mins at RT. Secondary Antibody: Anti-Rabbit Poly HRP-IgG Ready to Use for 8 mins at RT. Counter stain: Hematoxylin. Substrate: DAB. Results: shows moderate cytoplasmic staining in vascular endothelial cells, perivascular and peribronchiolar mononuclear cells and spindle cells (fibroblasts) at 1:100 dilution. There is intense cross reactivity with smooth muscle sounding the bronchi and bronchioles.

product information

Catalog Number :

600-401-MN2

Name :

Anti-Cxcl1 (RABBIT) Antibody - 600-401-MN2

Display Name :

Cxcl1 Antibody

Application Note :

Anti-Cxcl1 Antibody has been tested in ELISA, WB, and IHC. Expect a band at ~10.3kDa in western blot using appropriate tissues and lysates. Positive control used: mouse spleen and lung in Western blot and immunohistochemistry.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000-1:50,000

Immunohistochemistry Dilution :

1:100

Western Blot Dilution :

1-5.0 µg/ml

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

Cxcl1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-Cxcl1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal portion of mouse Cxcl1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against mouse Cxcl1. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to mouse.

Species Reactivity :

Mouse

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

Growth-regulated alpha protein, C-X-C motif chemokine 1, Platelet-derived growth factor-inducible protein KC, Secretory protein N51, KC(5-72), Hematopoietic synergistic factor, HSF, KC-T, Cxcl1, Gro, Gro1, Mgsa, Scyb1

Target Species :

Mouse

Background :

Cxcl1 encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. It has chemotactic activity for neutrophils and contributes to neutrophil activation during inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. KC(5-72) shows a highly enhanced hematopoietic activity. Anti-Cxcl1 Antibody is useful for researchers interested in Kaposi Sarcoma, H. Pylori Infections, CCR5 and NOD-like receptor pathways, and Chemokine activities.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

Primary Image Name :

Immunohistochemistry of Rabbit Anti-Cxcl1 Antibody

New Product :

Yes

more info or order :

Rockland Immunochemicals product webpage