Foxp3 Antibody | Rockland Immunochemicals 600-401-MN0S product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Foxp3 Antibody

catalog :

600-401-MN0S

quantity :

25 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, flow cytometry

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

Flow Cytometry of Rabbit Anti-Foxp3 Antibody. Cells: NIH-3T3 cells. Treatment: Fix/Perm solution for 30 minutes at 4°C and washed twice in Permeabilization Buffer. Primary Antibody: Anti-Foxp3 4µL in 100uL FACS buffer for 30 min at room temperature. Secondary Antibody: F(ab')2 Donkey Anti-Rabbit IgG Phycoerythrin (p/n 711-708-127) 5µL of secondary antibody in 100µL of reaction (used for for intra-nuclear staining). Isotype control: Rabbit Anti-Rad9 pS1129 Antibody (p/n 600-401-345).

product information

Catalog Number :

600-401-MN0S

Name :

Anti-Foxp3 (RABBIT) Antibody - 600-401-MN0S

Display Name :

Foxp3 Antibody

Application Note :

Anti-Foxp3 Antibody has been tested in ELISA, WB, IF, and FLOW. Expect a band for isoforms 1 and 4 at ~47.2 and 44.4kDa in western blot using appropriate tissues and lysates. Positive control used: NIH/3T3 cells in Immunofluorescence and Flow cytometry.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

0.99 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

25 µL

Default Unit :

ELISA Dilution :

1:10,000-1:50,000

Flow Cytometry Dilution :

1:25

IF Microscopy Dilution :

15µg/mL

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

Foxp3

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-Foxp3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-terminal portion of mouse Foxp3 conjugated to Keyhole Limpet Hemocyanin (KLH).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against mouse Foxp3. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to mouse, human, and Macaca fascicularis.

Species Reactivity :

human, mouse

Storage :

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Synonyms :

Rabbit Anti-Forkhead Box P3 Antibody, Immune Dysregulation Polyendocrinopathy Enteropathy, X-Linked, Forkhead Box Protein P3, Scurfin, IPEX, Immunodeficiency Polyendocrinopathy Enteropathy X-Linked, FOXP3delta7, DIETER, AIID, PIDX, XPID, JM2, Forkhead box protein P3 41 kDa form, Forkhead box protein P3 C-terminally processed

Target Species :

Mouse

Background :

FOXP3 is a member of the forkhead/winged-helix family of transcriptional regulators. The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. FOXP3 acts as a repressor of transcription and regulates T cell activation, with its overexpression in CD4 T cells leading to an attenuation of activation-induced cytokine production and proliferation. In regulatory T (Treg) cells, FOXP3 is essential for Treg suppressor function and its expression leads to the repression of IL-17 expression. Mutations in FOXP3 gene cause IPEX, a fatal, X-linked inherited disorder characterized by immune dysregulation. The FOXP3 protein is essential for normal immune homeostasis. Specifically, FOXP3 represses transcription through a DNA binding forkhead domain, thereby regulating T cell activation. Anti-FOXP3 antibody is useful for researchers interested in transcription factor activity, polyendocrinopathy, immunodysregulation, and DNA binding research.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Yes

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Flow Cytometry,IF Microscopy,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q99JB6

NCBI :

NP_001186276.1

Primary Image Name :

Flow Cytometry of Rabbit Anti-Foxp3 Antibody

New Product :

Yes

more info or order :

Rockland Immunochemicals product webpage