SNAI1 Antibody | Rockland Immunochemicals 600-401-MM5 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

SNAI1 Antibody

catalog :

600-401-MM5

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

Immunofluorescence of Rabbit Anti-SNAI1 Antibody. Cell Line: NIH-3T3 cells. Fixative: 4% PFA. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-SNAI1 at 15µg/mL overnight at 2-8°C. Secondary Antibody: Donkey Anti-Rabbit IgG DyLight™488 (p/n 611-741-127) at 5µL/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A). DAPI. (B). SNAI1 + DyLight™488 secondary. (C). Merge A+B. (D). secondary only. Localization expected: nucleus, cytosol.

product information

Catalog Number :

600-401-MM5

Name :

Anti-SNAI1 (RABBIT) Antibody - 600-401-MM5

Display Name :

SNAI1 Antibody

Application Note :

Anti-SNAI1 Antibody has been tested in WB, IHC, and IF. Expect a band at ~29 in western blot using appropriate lysates. Positive control used: SNAI1 overexpressed HEK293 in WB, human lung tissue in IHC, NIH-3T3 and HeLa in IF.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.03 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000-1:50,000

Immunohistochemistry Dilution :

1:300

IF Microscopy Dilution :

15µg/mL

Western Blot Dilution :

1:2000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

SNAI1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-SNAI1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human SNAI1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Other Performance Data :

User Optimized

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against human SNAI1. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to human and mouse.

Species Reactivity :

Human, Mouse

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

Rabbit Anti-SNAI1 Antibody, Snail Family Transcriptional Repressor 1, Snail Family Zinc Finger 1, Zinc Finger Protein SNAI1, Protein Snail Homolog 1, Protein Sna, SNAH, Snail 1 (Drosophila Homolog), Zinc Finger Protein, Snail Homolog 1 (Drosophila), Snail 1 Zinc Finger Protein, Snail 1 Homolog, Snail Homolog 1, DJ710H13.1, SLUGH2, SNAIL1, SNAIL, SNA

Target Species :

Human

Background :

SNAI1 (Snail Family Transcriptional Repressor 1) is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. SNAI1 is involved in the induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. It binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. During EMT, SNAI1 is involved with LOXL2 in negatively regulating pericentromeric heterochromatin transcription (By similarity). SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits (By similarity). It associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, SNAI1 may also activate the CDKN2B promoter by itself. SNAI1 may play a role in Gastroesophageal Junction Adenocarcinoma and Fasciolopsiasis. Anti-SNAI1 Antibody is useful for researchers interested in Cytoskeleton remodeling Regulation of actin cytoskeleton by Rho GTPases and Kit receptor signaling pathways.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Immunohistochemistry,IF Microscopy,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

O95863

NCBI :

NP_005976.2

Primary Image Name :

Immunofluorescence of Rabbit Anti-SNAI1 Antibody

more info or order :

Rockland Immunochemicals product webpage