MeCP2 Antibody | Rockland Immunochemicals 600-401-MM0 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

MeCP2 Antibody

catalog :

600-401-MM0

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

Immunohistochemistry of Rabbit Anti-MeCP2 Antibody. Tissue: Human Tonsil. Primary Antibody: Anti-MeCP2 Antibody at 1:200 for 30 min at RT. Secondary Antibody: Anti-Rabbit Poly-HRP IgG Ready-to-Use for 8 min at RT. Antigen Retrieval: HIER citrate buffer for 20 min. Counterstain: Hematoxylin. Staining: MeCP2 showed strong nuclear staining in human tonsil while showing less staining in germinal centers. This is consistent with the pattern seen in databases. The pattern is seen most clearly at 1:200 dilution. A concentration of 1:200 or lower is recommended.

product information

Catalog Number :

600-401-MM0

Name :

Anti-MeCP2 (RABBIT) Antibody - 600-401-MM0

Display Name :

MeCP2 Antibody

Application Note :

Anti-MeCP2 Antibody has been tested in ELISA, Western Blot, and Immunohistochemistry. Expect a band at ~52 kDa in western blot using appropriate lysates or tissues. Positive control used U2-OS, A431, human cerebellum tissue, and Chicken Spinal Cord in WB, human tonsil tissue in IHC.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000-1:15,000

Immunohistochemistry Dilution :

1:200

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

MECP2

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-MeCP2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-terminal portion of human MeCP2 conjugated to Keyhole Limpet Hemocyanin (KLH).

Other Performance Data :

This antibody is not suitable for CHIP.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against human MeCP2. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to mouse, rat, and macaca fascicularis.

Species Reactivity :

human, chicken

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

Rabbit Anti-Methyl-CpG Binding Protein 2 Antibody, Rabbit-Anti-MeCP2 Antibody, MeCp-2 Protein, Methyl CpG Binding Protein 2 (Rett Syndrome), Testis Tissue Sperm-Binding Protein Li 41a, Mental Retardation, X-Linked 16, Mental Retardation, X-Linked 79, Methyl-CpG-Binding Protein 2, Methyl CpG Binding Protein 2, Rett Syndrome, AUTSX3, MRXS13, MRXSL, MRX16, MRX79, MeCp2, PPMX, RTS, RTT, RS

Target Species :

Human

Background :

MeCP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. MECP2 mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. It binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC). Anti-MeCP2 Antibody is useful for researchers interested in Rett Syndromes, SIDS, Mental Retardation X-Linked Syndromic 13, and chromatin binding pathways.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P51608

NCBI :

NP_001104262.1

Primary Image Name :

Immunohistochemistry of Rabbit Anti-MeCP2 Antibody

more info or order :

Rockland Immunochemicals product webpage