product summary
Loading...
company name :
Rockland Immunochemicals
product type :
antibody
product name :
SLC40A1 Antibody
catalog :
600-401-MG1
quantity :
100 µg
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA
more info or order :
image
image 1 :

Immunofluorescence of Rabbit Anti-SLC40A1 Antibody.
Cells: Hep-G2 Cells.
Fixative: 4% PFA.
Permeabilization: 0.3% Triton X-100.
Primary Antibody: Anti-SLC40A1 at 15µg/mL overnight at 2-8°C.
Counterstain: Donkey Anti-Rabbit IgG DyLight™488 (p/n 611-741-127) at 5µg/mL for 1hr at RT.
Nuclear Counterstain: DAPI.
Staining: (A) DAPI. (B) Anti-SLC40A1 + DyLight™488. (C) Merge A + B. (D) secondary only.
Expected Localization: cytoplasm/plasma.
product information
Catalog Number :
600-401-MG1
Name :
Anti-SLC40A1 (RABBIT) Antibody - 600-401-MG1
Display Name :
SLC40A1 Antibody
Application Note :
Anti-SLC40A1 Antibody has been tested in ELISA, WB, and IF. Expect a band at ~62.5kDa in western blot using appropriate lysates. Positive control used: Caco-2 lysate in Western Blot and Hep-G2 in Immunofluorescence.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
1.04 mg/ml
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
(None)
Size :
100 µg
Default Unit :
µg
ELISA Dilution :
1:10,000 - 1:50,000
IF Microscopy Dilution :
15 µg/ml
Western Blot Dilution :
10 µg/ml
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG
Gene Name :
SLC40A1
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-SLC40A1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human SLC40A1 conjugated to Keyhole Limpet Hemocyanin (KLH).
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
This affinity purified antibody is directed against human SLC40A1. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to mouse and rat.
Species Reactivity :
Human
Storage :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Synonyms :
Rabbit Anti-SLC40A1 Antibody, Solute Carrier Family 40 Member 1, Solute Carrier Family 11 (Proton-Coupled Divalent Metal Ion Transporters), Member 3, Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1, SLC11A3, IREG1, FPN1, Iron-Regulated Transporter 1, Iron Regulated Gene 1, Ferroportin-1, Ferroportin 1, MSTP079, MST079, HFE4, MTP1
Target Species :
Human
Background :
SLC40A1 (Solute Carrier Family 40 Member 1) may be involved in iron export from duodenal epithelial cell and in transfer of iron between maternal and fetal circulation. Mediates iron efflux in the presence of a ferroxidase (hephaestin and/or ceruloplasmin). Defects in this gene are a cause of hemochromatosis type 4 (HFE4). Diseases associated with SLC40A1 include Hemochromatosis Type 4 and Type 1. Anti-SLC40A1 Antibody is useful for researchers interested in mineral absorption, insulin receptor recycling, and transmembrane transporter activity.
Immunogen Type :
Peptide
Low Endotoxin :
No
Sample Size :
No
Target Domain of Reactivity :
Internal
Application Text :
ELISA,IF Microscopy,Western Blot,
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
UniProt :
Q9NP59
NCBI :
NP_055400.1
Primary Image Name :
Immunofluorescence of Rabbit Anti-SLC40A1 Antibody
New Product :
Yes
more info or order :
company information

Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.
related products
browse more products
questions and comments