p35 Antibody | Rockland Immunochemicals 600-401-ME9 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

p35 Antibody

catalog :

600-401-ME9

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

Immunohistochemistry of Rabbit Anti-p35 Antibody. Tissue: Mouse Brain. Fixative: none. Antigen Retrieval: HIER. Primary Antibody: Anti-p35/Cdk5 Antibody at 1:100. Secondary Antibody: Ready-to-Use Anti-Rabbit. Counterstain: Hematoxylin. Results: p35 developed a strong nuclear and membranous staining of neurons and neurophil in brain tissue.

product information

Catalog Number :

600-401-ME9

Name :

Anti-p35/Cdk5 (RABBIT) Antibody - 600-401-ME9

Display Name :

p35 Antibody

Application Note :

Anti-p35/Cdk5 Antibody has been tested in ELISA, WB, IF, and IHC. Expect bands ~34 kDa in western blot using appropriate tissues and lysates. Positive control used: mouse brain lysate in WB and IHC.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

0.96 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000 - 1:50,000

Immunohistochemistry Dilution :

1:100

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

Cdk5r1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-p35/Cdk5 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-terminal portion of mouse p35/Cdk5 conjugated to Keyhole Limpet Hemocyanin (KLH).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against mouse p35/Cdk5. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to rat, human, bovine, and Spermophilus citellus.

Species Reactivity :

human, mouse

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

Rabbit Anti-Cyclin Dependent Kinase 5 Regulatory Subunit 1 Antibody, Rabbit Anti-Cyclin-dependent kinase 5 activator 1 Antibody, Rabbit Anti-p35 Antibody, TPKII Regulatory Subunit, CDK5 Activator 1, CDK5R, NCK5A, Cyclin-Dependent Kinase 5, Regulatory Subunit 1 (P35), Cyclin-Dependent Kinase 5 Regulatory Subunit 1, Cyclin-Dependent Kinase 5 Activator 1, Tau Protein Kinase II 23kDa Subunit, Regulatory Partner For CDK5 Kinase, Neuronal CDK5 Activator, P35nck5a, CDK5P35, P23, P25, P35

Target Species :

Mouse

Background :

p35, is a neuron specific activator of CDK5. Also known as Cdk5r1 (Cyclin-dependent kinase 5 activator 1), the complex p35/CDK5 is required for neurite outgrowth and cortical lamination. It is involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. Activator of TPKII. The complex p35/CDK5 participates in the regulation of the circadian clock by modulating the function of CLOCK protein: phosphorylates CLOCK at Thr-451 and Thr-461 and regulates the transcriptional activity of the CLOCK-ARNTL/BMAL1 heterodimer in association with altered stability and subcellular distribution. Diseases associated with CDK5R1 include Alzheimer Disease and Non-Syndromic Intellectual Disability. Anti-p35/Cdk5 Antibody is useful for researchers interested in Alzheimer researcher, protein kinase binding, Neuroscience Research, and Cancer Research.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

N-Terminus

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P61809

NCBI :

NP_034001.1

Primary Image Name :

Immunohistochemistry of Rabbit Anti-p35 Antibody

more info or order :

Rockland Immunochemicals product webpage