Histone H3 K36me3 Antibody | Rockland Immunochemicals 600-401-I88 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Histone H3 K36me3 Antibody

catalog :

600-401-I88

quantity :

50 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-I88

Name :

Anti-Histone H3 [Trimethyl Lys36] (RABBIT) Antibody - 600-401-I88

Display Name :

Histone H3 K36me3 Antibody

Application Note :

Anti-Histone H3 [Trimethyl Lys36] antibody is useful for Western Blot, Chromatin Immunoprecipitation, Dot Blot, and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

50 µg

Default Unit :

µg

Immunohistochemistry Dilution :

1:500

IF Microscopy Dilution :

1:500

Western Blot Dilution :

1:500

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

HIST2H3C

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Histone H3 [Trimethyl Lys36] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic trimethylated peptide surrounding Lysine 36 of human Histone H3.2.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-Histone H3 [Trimethyl Lys36] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

Species Reactivity :

Human, mouse, C. elegans

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-Histone H3 trimethyl Lys36 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3K36me3

Target Species :

Human

Background :

The histone modification H3K36me3 is important in the differentiation and maintenance of specialized cells derived from stem cell progenitors. The presence and abundance of H3K36me3 is correlated to the downstream transcription of those pathway components important for cellular functions that differentiate cells from each other. The polycomb repressive complex 2 (PRC2) represses transcription by methylation of H3 lysine 27, but this methylation is inhibited by the presence of the H3K36me3 mark. SETD2 is the main methyltransferase responsible for methylating the H3K36me3. Once methylated, H3K36me3 is highly associated with active transcription factors, and the generation of integral downstream pathways, which move stem cells towards differentiation. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.

Immunogen Type :

Peptide

Low Endotoxin :

Post Translational Modification :

Methylation

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

Immunohistochemistry,IF Microscopy,Western Blot,

Other :

Dot Blot 0.5µg/mL

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q71DI3

NCBI :

NP_001005464

Primary Image Name :

Histone H3 [Trimethyl Lys36] Western Blot

more info or order :

Rockland Immunochemicals product webpage