Cytochrome P450 Antibody | Rockland Immunochemicals 600-401-HB7 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Cytochrome P450 Antibody

catalog :

600-401-HB7

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

Immunofluorescence of Rabbit Anti-Cytochrome p450 Antibody. Cell Line: HepG2. Fixation: 4% PFA. Permeabilization: 0.3%Triton X-100. Primary Antibody: Rabbit Anti-Cytochrome p450 Antibody at 15µg/mL overnight at 4°C. Secondary Antibody: Donkey Anti-Rabbit DyLight™ 488 Conjugated Antibody (p/n 611-741-127) at 5µg/mL for 1hr at RT. A: Nuclear counter stain: DAPI. B: Anti-Cytochrome p450 and Anti-Rabbit DyLight™ 488. C: Actin- Phalloidin555. D: Secondary Only. E: Merge A+B. F: Merge A+B+C.

product information

Catalog Number :

600-401-HB7

Name :

Anti-Cytochrome P450 (RABBIT) Antibody - 600-401-HB7

Display Name :

Cytochrome P450 Antibody

Application Note :

Anti-Cytochrome p450 has been tested in Western Blot, Immunofluorescence and Immunohistochemistry. Expect a band at ~58kda in western blot using appropriate lysates. Positive controls used include an overexpressing lysate and PC-12 at 1µg/mL for WB; human kidney and/or lung for IHC; HepG2 for IF at 15 µg/mL.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

5.0 ug/ml

Immunohistochemistry Dilution :

1:100 - 1:200

IF Microscopy Dilution :

15 ug/ml

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

CYP1A1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-Cytochrome p450 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human cytochrome p450 conjugated to Keyhole Limpet Hemocyanin (KLH).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against human cytochrome p450. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis the immunogen sequence shows 83.3% identity with mouse, rat, hamster, and monkey.

Species Reactivity :

human, rat

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

Rabbit Anti-Cytochrome p450 Antibody, Rabbit Anti-CYP1A1 Antibody, CYPIA1, Cytochrome P450 form 6, Cytochrome P450-C, Cytochrome P450-P1

Target Species :

Human

Background :

Cytochrome p450 belongs to an enzyme superfamily that catalyzes many reactions such as the synthesis of cholesterol, lipids, steroids by oxidizing the compounds and plays a role in drug metabolism. The protein has been known to be stimulated by cigarette smoke. On-going studies link the cytochrome p450 protein with lung cancer. In the liver microsome, it is part of the NADPH-dependent electron transport pathway. The protein has been found expressed in the lung, lymphocytes and placenta. Anti-Cytochrome p450 antibody is useful for researchers interested in cancer research and enzyme research.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Immunohistochemistry,IF Microscopy,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P04798

NCBI :

NP_000490.1

Primary Image Name :

Immunofluorescence of Rabbit Anti-Cytochrome p450 Antibody

more info or order :

Rockland Immunochemicals product webpage