TRIM5 alpha Antibody | Rockland Immunochemicals 600-401-H28 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

TRIM5 alpha Antibody

catalog :

600-401-H28

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, rhesus macaque

application :

western blot

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-H28

Name :

Anti-TRIM5 alpha (RABBIT) Antibody - 600-401-H28

Display Name :

TRIM5 alpha Antibody

Application Note :

Anti-TRIM5 alpha Antibody is suitable for use in E, Wb and IF. Expect a band approximately ~356.3 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

Western Blot Dilution :

0.5-2 ug/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

TRIM5

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

TRIM5 alpha Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to amino acids near the C-terminus of rhesus monkey TRIM5 alpha.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-TRIM5 alpha Antibody was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with TRIM5 alpha with Human based on 100% homology with the immunizing sequence. Cross-reactivity with TRIM5 alpha from other sources has not been determined. Anti-TRIM5 alpha is human specific but should presumably also react with rhesus protein.

Species Reactivity :

Human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

RNF88

Target Species :

Monkey

Background :

Trim5 antibody detects rhesus monkey Trim5 alpha. TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has six alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5 alpha variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. All TRIM5 alpha; variants could inhibit at least two different retroviruses, but not from those viruses isolated from the same species, suggesting that TRIM5 alpha acts as a natural barrier to cross-species retrovirus transmission. Anti-TRIM5 alpha is ideal for investigators involved in infectious disease research.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q0PF16

NCBI :

NP_001028082.1

more info or order :

Rockland Immunochemicals product webpage