Spred1 Antibody | Rockland Immunochemicals 600-401-H19 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Spred1 Antibody

catalog :

600-401-H19

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-H19

Name :

Anti-Spred1 (RABBIT) Antibody - 600-401-H19

Display Name :

Spred1 Antibody

Application Note :

Anti-Spred1 Antibody is suitable for use in E,WB, and IHC. Expect a band approximately ~50.4 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

Western Blot Dilution :

1-2 ug/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

SPRED1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Spred1 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a peptide corresponding to an internal region of the human Spred1.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-Spred1 Antibody was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with Spred1 with Human, Rat and Mouse based on 100% homology with the immunizing sequence. Cross-reactivity with Spred1 from other sources has not been determined. This Spred1 antibody is predicated to have no cross reactivity to Spred2 and Spred 3.

Species Reactivity :

Human, Mouse, Rat

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

Sprouty-related EVH1 domain-containing protein 1, NFLS

Target Species :

Human

Background :

Spred1 antibody detects human Spred1. The Ras-MAP kinase pathway is essential for the differentiation of neuronal cells and myocytes; it is inhibited by Spred1, a member of the Sprouty family of proteins. Spred1 acts by suppressing the phosphorylation and activation of Raf. The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Further studies have shown that expression levels of Spred1 and Spred2 proteins are inversely correlated with the incidence of tumor invasion and metastasis in human hepatocellular carcinoma (HHC), suggesting that these proteins could be useful as prognostic factors and therapeutic targets in HCC. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS). Anti-Spred1 antibodies are ideal for investigators involved in cell cycle proteins and cancer research.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q7Z699

NCBI :

NP_689807.1

more info or order :

Rockland Immunochemicals product webpage