Retinoblastoma K860 Me1 Antibody | Rockland Immunochemicals 600-401-GZ4 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Retinoblastoma K860 Me1 Antibody

catalog :

600-401-GZ4

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-GZ4

Name :

Anti-Retinoblastoma [Monomethyl Lys860] (RABBIT) Antibody - 600-401-GZ4

Display Name :

Retinoblastoma K860 Me1 Antibody

Application Note :

Anti-Retinoblastoma K860 Me1 Antibody was tested for use in Dot Blot, and Western Blot. Expect a band at approximately 106.1kDa in MOLT-4 and other appropriate cell lysates or tissues. Although not tested, expect reactivity in ELISA, immunoprecipitation and immunohistochemistry.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

0.99 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000-1:50,000

Western Blot Dilution :

1.0 ug/ml

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

RB1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-Retinoblastoma [Monomethyl Lys860] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a methylated and acetylated synthetic peptide surrounding lysine 860 of human RB1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Other Performance Data :

Dot blot: 1.0 ug/ml

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against human Retinoblastoma. This product was affinity purified from monospecific antiserum by immunoaffinity purification. This antibody reacts with human RB1 surrounding aa860 and is specific for K860 Me1. Blast analysis of the sequence of the immunogen shows 100% identity with human and 92% identity to rat and mouse.

Species Reactivity :

human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

Retinoblastoma-associated protein, p105-Rb, pRb, pp110, RB Transcriptional Co-repressor, Protein Phosphatase 1 Regulatory Subunit 130, Prepro-Retinoblastoma-Associated Protein, Retinoblastoma, P105-Rb, Retinoblastoma Susceptibility Protein, Retinoblastoma-Associated Protein, Osteosarcoma, PPP1R130, OSRC

Target Species :

Human

Background :

RB1 (RB Transcriptional Corepressor 1) is a protein coding gene. The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The active, hypo-phosphorylated form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. RB1 is directly involved in heterochromatin formation by maintaining overall chromatin structure and the constitutive heterochromatin, by stabilizing histone methylation. Retinoblastoma recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. It controls histone H4 Lys-20 trimethylation and inhibits the intrinsic kinase activity of TAF1. It mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein, or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1s activity. RB1 may be associated with diseases such as retinoblastoma, small cell cancer of the lung, bladder cancer, and osteogenic sarcoma. Anti-Retinoblastoma K860 Me1 Antibody is useful for researchers interested in cancer, transcription factor, epigenetics, and enzyme antibody research.

Immunogen Type :

Peptide

Low Endotoxin :

Post Translational Modification :

Methylation

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

ELISA,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P06400

NCBI :

NP_000312.2

Primary Image Name :

Western Blot of Rabbit anti-Retinoblastoma [Monomethyl Lys860] antibody

more info or order :

Rockland Immunochemicals product webpage