ASAP1 Antibody | Rockland Immunochemicals 600-401-GY1 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

ASAP1 Antibody

catalog :

600-401-GY1

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-GY1

Name :

Anti-ASAP1 (RABBIT) Antibody - 600-401-GY1

Display Name :

ASAP1 Antibody

Application Note :

Anti-ASAP1 Antibody has been tested for use in ELISA and Western Blot. Expect a band approximately ~125kDa using positive control lysates over-expressing ASAP1, or other specific lysates or tissues. Although not tested, this antibody could be useful in Immunofluorescence or Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.04 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:1000 - 1:5000

Western Blot Dilution :

1.0 ug/ml

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

ASAP1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-ASAP1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the c-terminus region of human ASAP1 protein.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against human ASAP1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity to human 100%, bovine 93%, and rat and mouse 87% homology for the immunogen sequence. Cross-reactivity from other sources has not been determined.

Species Reactivity :

human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-ASAP1, rabbit anti ASAP1, Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1, 130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein, ADP-ribosylation factor-directed GTPase-activating protein 1, ARF GTPase-activating protein 1, Development and differentiation-enhancing factor 1, DEF-1, Differentiation-enhancing factor 1, PIP2-dependent ARF1 GAP

Target Species :

Human

Background :

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI). ASAP1 (ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 1) is a Protein Coding gene. This gene encodes an ADP-ribosylation factor (ARF) GTPase-activating protein. The GTPase-activating activity is stimulated by phosphatidylinositol 4,5-biphosphate (PIP2), and is greater towards ARF1 and ARF5, and lesser for ARF6. This gene maybe involved in regulation of membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. ASAP1 may function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types (By similarity) and plays a role in ciliogenesis. Anti-ASAP1 is useful for researchers interested in GTPase activities and EGFR1 Signaling Pathway.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

ELISA,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q9ULH1

NCBI :

NP_001234925.1

Primary Image Name :

Western blot of Anti-ASAP1

more info or order :

Rockland Immunochemicals product webpage