Ffar4 Antibody | Rockland Immunochemicals 600-401-GW0 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Ffar4 Antibody

catalog :

600-401-GW0

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

mouse, rat

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-GW0

Name :

Anti-Ffar4 (RABBIT) Antibody - 600-401-GW0

Display Name :

Ffar4 Antibody

Application Note :

Anti-Ffar4 Antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~40-48 kDa in size corresponding to Ffar4 by western blotting in the appropriate cell lysate or extract. Tested using positive controls Raw 264.7 WCL p/n W10-001-369 and NIH/3T3 WCL p/n W10-000-358.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.08 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

5.0 ug/ml

Western Blot Dilution :

1.0 ug/ml

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

Ffar4

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Affinity purified Anti-Ffar4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the N-terminus portion of mouse Ffar4 protein.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-Ffar4 is directed against mouse Ffar4 at a n-terminal position. This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads. A BLAST analysis was used to suggest reactivity with this protein in rat based on 100% homology match for the immunogen sequence.

Species Reactivity :

Mouse, Rat

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-Ffar4 antibody, Ffar-4, Ffar 4, free fatty acid receptor 4, omega-3 fatty acid receptor 1, G-protein coupled receptor 120, G-protein coupled receptor GT01, Gpr120, O3far1

Target Species :

Mouse

Background :

Anti-Ffar4 Antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Free Fatty Acid 4 receptor (Ffa4 receptor or GPR120), a rhodopsin-like G protein coupled receptor (GPCR) subfamily member, is a receptor that senses specific fatty acids such as omega-3 fatty acid in fish oil or the endogenous signaling lipid, PHASA. Ffa4 receptor is enriched in lung, colon and adipose tissue but is also detected in many other tissues and cells. The activation of Ffar4 has multiple effects, including but not limited to inhibition of inflammation, improving insulin sensitivity and adipogenesis, and regulating hormone secretion from the gastro-intestinal system and pancreatic islets. Therefore, approaches that regulate FFA4 receptor activity could be developed as promising anti-diabetic and anti-inflammation drugs. GPR120 is the only fatty acid receptor that can sense lipids in adipose tissue, mature adipocytes, CD11c+ macrophages, and RAW264.7 cells making this receptor of potential importance in the prevention and treatment of metabolic and inflammatory diseases.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

N-Terminus

Application Text :

ELISA,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q7TMA4

NCBI :

NP_861413.1

Primary Image Name :

Western blot of Anti-Ffar4 Antibody

more info or order :

Rockland Immunochemicals product webpage