ATF6 Antibody | Rockland Immunochemicals 600-401-GU9 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

ATF6 Antibody

catalog :

600-401-GU9

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

Immunofluorescence Microscopy of Rabbit anti-ATF6 antibody. Tissue: U-251 cells p/n (W09-001-GY4). Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: ATF6 antibody at 10 µg/mL overnight at 4⁰C. Secondary antibody: Anti-RABBIT IgG DyLight™ 488 Conjugated Preadsorbed (p/n 611-741-127) at 5 ug/ml for 2 h at RT. Localization: ATF6 is cell membrane and cytoplasmic. Staining: ATF6 as green fluorescent signal with DAPI (blue) nuclear counterstain.

product information

Catalog Number :

600-401-GU9

Name :

Anti-ATF6 (RABBIT) Antibody - 600-401-GU9

Display Name :

ATF6 Antibody

Application Note :

Anti-ATF6 Antibody has been tested in Western Blot, IF and ELISA. Recombinant ATF6 protein positive control was used for western blotting. Expect a band at 74.5kDa in western blot using appropriate lysates or recombinant protein. Antibody tested positive in U251 cells (W09-001-GY4) in Immunofluorescence. Although not tested, this antibody is likely functional in immunohistochemistry, flow cytometry, and immunoprecipitation.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

5.0 ug/ml

Flow Cytometry Dilution :

User Optimized

Immunohistochemistry Dilution :

1:200

IF Microscopy Dilution :

10 ug/ml

Immunoprecipitation Dilution :

User Optimized

Western Blot Dilution :

0.5 ug/ml

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

ATF6

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-ATF6 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Term portion of human ATF6 conjugated to Keyhole Limpet Hemocyanin (KLH).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against human ATF6. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with human and 80% identity to rat.

Species Reactivity :

human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-ATF6 Antibody, Activating Transcription Factor 6, rabbit anti-ATF-6 Antibody, Cyclic AMP-dependent transcription factor ATF-6 alpha, Activating transcription factor 6 alpha, ATF6 alpha

Target Species :

Human

Background :

ATF6 belongs to the bZIP (basic-leucine zipper domain transcription factor) family. Activating target genes for the unfolded protein response during endoplasmic reticulum stress. Embeded in the endoplasmic reticulum, it functions as an ER stress sensor/stress transducer. Following stress-induced proteolysis it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. Binds DNA on the 5'-CCAC[GA]-3' half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. ATF6 could also be involved in the activation of transcription by the serum response factor. This protein may play a role in the development of the foveal and cone function in the retina. Therefore, attributing to disorders such as achromatopsia, blindness, nystagmus, myopia, cataracts and more. Anti-ATF6 Antibody is useful for researchers studying eye development, protein folding and processing, Endoplasmic Reticulum Stress Pathways, Glucose and Energy Metabolism Research.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

N-Terminus

Application Text :

ELISA,Flow Cytometry,Immunohistochemistry,IF Microscopy,Immunoprecipitation,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P18850

NCBI :

NP_031374.2

Primary Image Name :

Immunofluorescence of Anti-ATF6 (RABBIT) Antibody

more info or order :

Rockland Immunochemicals product webpage