PINK1 truncated Antibody | Rockland Immunochemicals 600-401-GU5 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

PINK1 truncated Antibody

catalog :

600-401-GU5

quantity :

100 µg

price :

369.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry

more info or order :

Rockland Immunochemicals product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 2g	Chen P, Katsuyama E, Satyam A, Li H, Rubio J, Jung S, et al. CD38 reduces mitochondrial fitness and cytotoxic T cell response against viral infection in lupus patients by suppressing mitophagy. Sci Adv. 2022;8:eabo4271 pubmed publisher

image

image 1 :

Immunofluorescence Microscopy of Rabbit anti-PINK1 truncated antibody. Cell line: NIH 3T3 (p/n W10-000-358). Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: PINK1 truncated antibody at 20 µg/mL for overnight at 4°C. Secondary antibody: Anti-RABBIT IgG DyLight™ 488 Conjugated Preadsorbed (p/n 611-741-127) at 5 ug/ml for 2 hrs at RT. Localization: PINK1 is mitochondrial and cytoplasmic. Staining: PINK1 as green fluorescent signal with DAPI (blue) nuclear counterstain.

product information

Catalog Number :

600-401-GU5

Name :

Anti-PINK1 truncated (RABBIT) Antibody - 600-401-GU5

Display Name :

PINK1 truncated Antibody

Application Note :

Anti-PINK1 truncated Antibody has been tested in Western Blot, Immunofluorescence, and ELISA. Positive controls were recombinant PINK1 protein and overexpressed PINK1 transfected HEK293 @ 1µg/mL in western blot. Expect a lower strength band at 62.7 kDa in western blot using appropriate lysates. PINK1 truncated antibody strongly detects endogenously truncated PINK1 protein, seen at ~30kDa. PINK1 is highly phosphorylated and degrades rapidly which may cause additional banding in western blot. Positive control cell lines for immunofluorescence assays are NIH3T3 (p/n W10-000-358). Although not tested, this antibody is likely functional in immunohistochemistry, flow cytometry, and immunoprecipitation.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

User Optimized

Flow Cytometry Dilution :

User Optimized

Immunohistochemistry Dilution :

1:200

IF Microscopy Dilution :

20 µg/mL

Immunoprecipitation Dilution :

User Optimized

Western Blot Dilution :

1µg/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

PINK1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-PINK1 truncated antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Term portion of human PINK1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against human PINK1. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with human and 93% identity to mouse.

Species Reactivity :

Human, Mouse

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-PINK1 antibody, Serine/threonine-protein kinase PINK1 (mitochondrial), BRPK, PTEN-induced putative kinase protein 1, PTEN-induced kinase

Target Species :

Human

Background :

PINK1 (PTEN induced putative kinase 1) belongs to the protein kinase superfamily and Ser/Thr protein kinase family. PINK1 protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins. It is involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PARK2. Targets PARK2 to dysfunctional depolarized mitochondria through the phosphorylation of MFN2. Activates PARK2 in 2 steps: (1) by mediating phosphorylation at Ser-65 of PARK2 and (2) mediating phosphorylation of ubiquitin, converting PARK2 to its fully-active form. PINK1 interacts with PARK2 and FBXO7 and forms a complexes with PARK2 and PARK7. PINK1 is associated with Parkinson disease 6 (PARK6). Anti-PINK1 Antibody is useful for researcher interested in neurological research, neurodegenerative disorders, Pink/Parkin Mediated Mitophagy, and Parkinsons Disease Pathway.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

ELISA,Flow Cytometry,Immunohistochemistry,IF Microscopy,Immunoprecipitation,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2020 Price :

369.00 USD

UniProt :

Q9BXM7

NCBI :

NP_115785.1

Primary Image Name :

Immunofluorescence Microscopy of Rabbit anti-PINK1 antibody

more info or order :

Rockland Immunochemicals product webpage