BCL3 Antibody | Rockland Immunochemicals 600-401-GU4 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

BCL3 Antibody

catalog :

600-401-GU4

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-GU4

Name :

Anti-BCL3 (RABBIT) Antibody - 600-401-GU4

Display Name :

BCL3 Antibody

Application Note :

Anti-BCL-3 Antibody has been tested in Western Blot, ELISA, and Immunofluorescence. Expect a band at 47.5 kDa in western blot using appropriate lysates. Positive control used recombinant BCL3 protein, HEK293T overexpressing BCL3 or U87 whole cell lysates at 1µg/mL in WB; and U87MG with PFA at 10µg/mL in IF. Although not tested, this antibody is likely functional in immunohistochemistry, flow cytometry, and immunoprecipitation.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

0.99 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

5.0 ug/ml

Flow Cytometry Dilution :

User Optimized

Immunohistochemistry Dilution :

1:200

IF Microscopy Dilution :

10 ug/ml

Immunoprecipitation Dilution :

User Optimized

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

BCL3

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-BCL3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Term portion of human BCL3 conjugated to Keyhole Limpet Hemocyanin (KLH).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against human BCL3. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with human.

Species Reactivity :

human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-BCL3 antibody, rabbit anti-BCL-3 Antibody, BCL 3, B-cell lymphoma 3 protein, B-cell lymphoma 3 protein Antibody

Target Species :

Human

Background :

BCL3 belongs to the ANKRD family. The protein contains seven ankyrin repeats, which are closely related to those found in I kappa B proteins. BCL3 functions as a transcriptional co-activator, regulator of transcriptional activation, promotor of transcription, and inhibitor of nuclear translocation of NF-kappa B. It is a proto-oncogene identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. BCL3 contributes to the regulation of cell proliferation. Therefore, BCL3 may be associated with B-cell chronic lymphocytic leukemia, lymphoma, or other hematologic diseases. Anti-BCL3 Antibody is useful for researchers interested in Cancer Research, NF-Kappa B Pathway, and TNF Signaling Pathway.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

ELISA,Flow Cytometry,Immunohistochemistry,IF Microscopy,Immunoprecipitation,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P20749

NCBI :

NP_005169.2

Primary Image Name :

Immunofluorescence Microscopy of Rabbit anti-BCL3 antibody

more info or order :

Rockland Immunochemicals product webpage