xCT Antibody | Rockland Immunochemicals 600-401-GU3 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

xCT Antibody

catalog :

600-401-GU3

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-GU3

Name :

Anti-xCT (RABBIT) Antibody - 600-401-GU3

Display Name :

xCT Antibody

Application Note :

Anti-xCT Antibody has been tested in Western Blot, ELISA, Immunofluorescence and Flow cytometry. Expect a band at 55.4 kDa in western blot using appropriate lysates. Positive control used NIH-3T3, Hela, HCT-116, A549, or PC3 whole cell lysates at 0.1-1.0µg/mL for WB and PC3 with MeOH at 10µg/mL in IF. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.02 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

5.0 ug/ml

Flow Cytometry Dilution :

1:200

Immunohistochemistry Dilution :

1:200

IF Microscopy Dilution :

10 ug/ml

Immunoprecipitation Dilution :

User Optimized

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

SLC7A11

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-xCT antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human xCT conjugated to Keyhole Limpet Hemocyanin (KLH).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against human xCT. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with human and orangutan, and 88% identity to mouse.

Species Reactivity :

human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-xCT antibody, SLC7A11, Cystine/glutamate transporter, Amino acid transport system xc-, Calcium channel blocker resistance protein CCBR1, Solute carrier family 7 member 11

Target Species :

Human

Background :

xCT belongs to the amino acid-polyamine-organocation (APC) superfamily and L-type amino acid transporter (LAT) family. xCT gene encodes a member of a heteromeric, sodium-independent, anionic amino acid transport system that is highly specific for cysteine and glutamate. In this system, designated Xc(-), the anionic form of cysteine is transported in exchange for glutamate. Increased expression of this gene results in neuronal cell death. xCT may be associated with kaposi sarcoma, dyscalculia, cystinuria, spondylolsis, and anemia of prematurity. Anti-xCT Antibody is useful for researchers interested in Cancer Research, Glucose/Energy Metabolism Research, and Cell Surface Protein Interaction Research.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Flow Cytometry,Immunohistochemistry,IF Microscopy,Immunoprecipitation,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q9UPY5

NCBI :

NP_055146.1

Primary Image Name :

Immunofluorescence Microscopy of Rabbit anti-xCT antibody

more info or order :

Rockland Immunochemicals product webpage