product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
Pax7 Antibody
catalog :
600-401-GR7
quantity :
100 µg
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, ELISA
product information
Catalog Number :
600-401-GR7
Name :
Anti-Pax7 (RABBIT) Antibody - 600-401-GR7
Display Name :
Pax7 Antibody
Application Note :
Anti-Pax7 antibody has been tested in ELISA and Western Blot.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 54.9 kDa in size corresponding to Pax7 by western blotting in the appropriate cell lysate or extract.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
1.0 mg/ml
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
(None)
Size :
100 µg
Default Unit :
µg
ELISA Dilution :
1:10,000-1:40:000
Western Blot Dilution :
1.0 ug/ml
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG
Gene Name :
Pax7
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Pax7 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Pax7 protein.
Other Performance Data :
This lot tested positive for use in western blotting and ELISA assays but is not suitable for immunofluorescence.
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
Anti-Pax-7 was affinity purified from monospecific antiserum by immunoaffinity chromatography.  A BLAST analysis was used to suggest cross reactivity with Pax7 protein from mouse (100% homology) and human (66.7% homology) for the immunizing sequence. Cross-reactivity with Pax7 from other sources has not been determined.
Species Reactivity :
Mouse, human
Storage :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Synonyms :
rabbit anti-Pax7 antibody, Paired box protein Pax-7, Pax 7, Pax-7
Target Species :
Mouse
Background :
The Anti-Pax7 antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Paired box protein Pax-7 transcription factor plays a role in myogenesis through regulation of muscle precursor cells proliferation. These genes play critical roles during fetal development and cancer growth. It is expressed specifically during the development of the nervous and muscular system. Anti-Pax7 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.
Immunogen Type :
Peptide
Low Endotoxin :
No
Sample Size :
No
Target Domain of Reactivity :
Internal
Application Text :
ELISA,Western Blot,
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
UniProt :
P47239
NCBI :
NP_035169.1
Primary Image Name :
Western blot for Anti-Pax7
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.