BECLIN1 Antibody | Rockland Immunochemicals 600-401-G84 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

BECLIN1 Antibody

catalog :

600-401-G84

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-G84

Name :

Anti-BECLIN1 (RABBIT) Antibody - 600-401-G84

Display Name :

BECLIN1 Antibody

Application Note :

Anti-Beclin-1 Antibody is suitable for use in ELISA, Western Blot, Immunohistochemistry, and Immunofluorescence. Expect a band approximately ~51.8 kDa in western blot using positive control HEK293 whole cell lysates, mouse brain tissue for IHC and IF, or other specific lysates. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

Immunohistochemistry Dilution :

2 µg/mL

IF Microscopy Dilution :

20 µg/mL

Western Blot Dilution :

0.5-2 ug/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

BECN1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Beclin-1 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a peptide near the n-terminus of human Beclin-1.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-Beclin-1 Antibody was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity withBeclin-1 with Human, Mouse and Rat based on 100% homology with the immunizing sequence. Cross-reactivity with Beclin-1 from other sources has not been determined.

Species Reactivity :

Human, Mouse, Rat

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

GT197, BECLIN 1, BECLIN-1

Target Species :

Human

Background :

Beclin-1 antibody detects human Beclin-1. Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6, is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors. Bcl-2 can bind to Beclin-1 and inhibit Beclin-1-dependent autophagy in yeast and mammalian cells, suggesting that Bcl-2 functions as an anti-autophagy protein as well as an anti-apoptotic protein, which helps maintain autophagy at levels that are more compatible with cell survival rather than cell death. Anti-Beclin-1 antibodies are ideal for investigators involved in apoptosis and cancer research.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

N-Terminus

Application Text :

Immunohistochemistry,IF Microscopy,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q14457

NCBI :

NP_001139117.1

more info or order :

Rockland Immunochemicals product webpage