TSC2 Antibody | Rockland Immunochemicals 600-401-FR1 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

TSC2 Antibody

catalog :

600-401-FR1

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-FR1

Name :

Anti-TSC2 (RABBIT) Antibody - 600-401-FR1

Display Name :

TSC2 Antibody

Application Note :

Anti-TSC2 Antibody has been tested for use in ELISA, Western Blotting, Immunocytochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 201 kDa in Western Blots of specific cell lysates and tissues.

Buffer :

0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:20,000-1:40,000

IF Microscopy Dilution :

20 µg/mL

Western Blot Dilution :

2-4 µg/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

TSC2

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-TSC2 (NT) was prepared from whole rabbit serum produced by repeated immunizations with a 14 amino acid synthetic peptide from near the N-terminus of human TSC2.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.02% (w/v) Sodium Azide

Purity and Specificity :

Anti-TSC2 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with TSC2 from other sources has not been determined.

Species Reactivity :

Human, mouse

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

TSC2 Antibody, LAM, TSC4, Tuberin, Tuberous sclerosis 2 protein

Target Species :

Human

Background :

Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 complex decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing that overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth. At least three isoforms of TSC2 exist.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

N-Terminus

Application Text :

ELISA,IF Microscopy,Western Blot,

Other :

Immunocytochemistry: 10 µg/mL

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P49815

NCBI :

NP_000539.2

Primary Image Name :

Western Blot of TSC2 Antibody

more info or order :

Rockland Immunochemicals product webpage