Ready-To-Use SMAD3 Antibody | Rockland Immunochemicals 600-401-E70-RTU product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Ready-To-Use SMAD3 Antibody

catalog :

600-401-E70-RTU

quantity :

100 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

Ready-To-Use Rabbit Anti-SMAD3 antibody follows the canonical TGF-Beta signaling pathway. TGF-Beta dimers bind to a receptor thereby activating the pathway. The type I receptor then recruits and phosphorylates a receptor regulated SMAD (R-SMAD) .i.e. SMAD2 or SMAD3. The R-SMAD then binds to the common SMAD (coSMAD) i.e. SMAD4, and forms a heterodimeric complex. This complex then enters the cell nucleus and acts as a transcription factor.

product information

Catalog Number :

600-401-E70-RTU

Name :

Ready-To-Use Anti-SMAD3 (RABBIT) Antibody - 600-401-E70-RTU

Display Name :

Ready-To-Use SMAD3 Antibody

Application Note :

Ready-To-Use Anti-SMAD3 Antibody has been optimized and validated in western blot using 1:1000 dilution. This Anti-SMAD3 (RTU) Antibody is sufficient to run 10 western blots. Expect a band approximately 48.1 kDa in size corresponding to human Smad3 protein by western blotting in the appropriate tissue or cell lysate or extract. Although not tested, this antibody is likely functional in ELISA, immunoprecipitation, and immunohistochemistry. Optimal titers for these applications should be determined by the researcher.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

sufficient to run 10 immunoblot tests

Conjugation :

(None)

Size :

100 µL

Default Unit :

ELISA Dilution :

User Optimized

Immunohistochemistry Dilution :

User Optimized

Immunoprecipitation Dilution :

User Optimized

Western Blot Dilution :

1:1,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

SMAD3

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

SMAD3 Antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad3 protein surrounding amino acid residue 179.

Packing Type :

Wet Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

RTU Anti-SMAD3 Antibody is directed against human Smad3 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Reactivity occurs against human Smad3 protein corresponding to an internal region surrounding amino acid residue 179. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, mouse, rat, pig, dog, and marmoset based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.

Species Reactivity :

human

Stabilizer :

0.01% Bovine Serum Albumin (BSA), 25% (v/v) Glycerol

Storage :

Store vial at 2-8° C prior to opening. May aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to use.

Synonyms :

hMAD 3 antibody, hSMAD3 antibody, MADH3 antibody, MGC60396 antibody, Mothers against decapentaplegic homolog 3 antibody, Mothers against DPP homolog 3 antibody, Smad-3

Target Species :

Human

Background :

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Smad3 (also known as Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-beta superfamily. Activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the S-S-X-S motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the bone morphogenic protein or BMP pathway. Upon phosphorylation R-Smads are translocated into nucleus, where they regulate transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80% of all TGF-b-mediated response. All Rockland Immunochemical’s SMAD3 antibodies are affinity-purified to assure both high affinity and specificity. Rigorous quality control testing ensures that the finished product meets or exceeds out high standards for optimum performance in your assays.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Immunohistochemistry,Immunoprecipitation,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P84022

NCBI :

NP_005893

Primary Image Name :

SMAD3 Pathway

more info or order :

Rockland Immunochemicals product webpage