This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Rockland Immunochemicals
product type :
antibody
product name :
Tyrosine Hydroxylase phospho S19 Antibody
catalog :
600-401-E50
quantity :
100 µL
price :
345.00 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry
product information
Catalog Number :
600-401-E50
Name :
Anti-Tyrosine Hydroxylase pS19 (Rabbit) Antibody - 600-401-E50
Display Name :
Tyrosine Hydroxylase phospho S19 Antibody
Application Note :
Anti-Tyrosine Hydroxylase pS19 (Rabbit) antibody is suitable for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 60 kDa in size corresponding to the tyrosine hydroxylase protein phosphorylated at Ser19 in the appropriate cell lysate or extract.
Buffer :
0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Clonality :
Polyclonal
Concentration Value :
Titrated value sufficient to run approximately 10 mini blots.
Conjugation :
(None)
Size :
100 µL
Default Unit :
ul
Immunohistochemistry Dilution :
1:1000
Western Blot Dilution :
1:1000
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG
Gene Name :
TH
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-Tyrosine Hydroxylase pS19 Antibody was produced by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser19.
Packing Type :
Dry Ice
Physical State :
Liquid
Purity and Specificity :
Anti-Tyrosine Hydroxylase pS19 Antibody is directed against phosphorylated rat Tyrosine Hydroxylase protein. The antibody was affinity purified from monospecific antiserum by immunoaffinity purification and is specific for the phosphorylated protein. Reactivity is expected from mouse and human. Cross reactivity with Tyrosine Hydroxylase from other species has not been determined.
Species Reactivity :
human, mouse, rat
Stabilizer :
0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol
Storage :
Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Synonyms :
Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, TH
Target Species :
Rat
Background :
Tyrosine Hydroxylase pS19 Antibody detects phosphorylated Tyrosine Hydroxylase protein. Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse. TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling. The activity of TH is also regulated by phosphorylation. Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs.
General Reference :
Dunkley PR, Bobrovskaya L, Graham ME, Von Nagy-Felsobuki EI and Dickson PW (2004). Tyrosine hydroxylase phosphorylation: regulation and consequences. J Neurochem 91:1025-1043. Haycock JW, Ahn NG, Cobb MH and Krebs EG (1992). ERK1 and ERK2, two microtubule-associated protein 2 kinases, mediate the phosphorylation of tyrosine hydroxylase at serine-31 in situ. Proc Natl Acad Sci (USA) 89:2365-2369. Haycock JW, Bennett WF, George RJ and Waymire JC (1982). Multiple site phosphorylation of tyrosine hydroxylase. Differential regulation in situ by a 8-bromo-cAMP and acetylcholine. J Biol Chem 257:13699-13703. Jedynak JP, Ali SF, Haycock JW and Hope BT (2002). Acute administration of cocaine regulates the phosphorylation of serine-19,-31 and-40 in tyrosine hydroxylase. J Neurochem 82:382-388. Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y and Haycock JW (2001). Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567. Salvatore MF, Waymire JC and Haycock JW (2001). Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. J Neurochem 79:349-360. Witkovsky P, Gabriel R, Haycock JW and Meller E (2000). Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina. J Chem Neuroanat 19:105-116. Zhu MY, Klimek V, Haycock JW and Ordway GA (2000). Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44. Zhu MY, Klimek V, Dilley GE, Haycock JW, Stockmeier C, Overholser JC, Meltzer HY and Ordway GA (1999). Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression. Biol Psychiatry 46:1275-1286. Coultrap S, Buard I, Kulbe J, Dell'Acqua M and Bayer K (2010). CaMKII Autonomy Is Substrate-dependent and Further Stimulated by Ca2+/Calmodulin J. Biol. Chem., 285: 17930 – 1793.
Immunogen Type :
Peptide
Low Endotoxin :
No
Post Translational Modification :
Phosphorylation
Sample Size :
No
Target Domain of Reactivity :
Internal
Created On :
2/29/2012 7:41 PM
Modified On :
1/2/2017 10:14 AM
Application Text :
Immunohistochemistry,Western Blot,
Approved for General Distributors :
12/1/2014
Discontinued Item :
No
Division :
OEM
Launch Date :
3/1/2012
Other :
User Optimized
Vendor :
PhosphoSolutions
Status :
Active
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
2018 Price :
345.00 USD
UniProt :
P04177
NCBI :
AAA42258.1
Primary Image Name :
Western Blot - Tyrosine Hydroxylase phospho S19 Antibody
company information
Rockland Immunochemicals
P.O. Box 326
Gilbertsville, PA 19525
Info@rockland-inc.com
www.rockland-inc.com
1-610-369-1008
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.